相关实验视频

Updated: Jun 29, 2025

Organotypic Tissue Model Systems for Investigating Host-Pathogen Interactions In Vitro

Organotypic Tissue Model Systems for Investigating Host-Pathogen Interactions In Vitro

Published on: March 28, 2025

N-acyl homoserine乳信号调节与人类牙斑相关的细菌群体

Rakesh Sikdar¹, Mai V Beauclaire², Bruno P Lima³

¹Biotechnology Institute, University of Minnesota, Saint Paul, MN 55108, USA.

bioRxiv : the preprint server for biology

|April 1, 2024

概括

N-同类素乳 (AHLs) 调节牙斑中的细菌通信. 破坏AHL信号改变了微生物群落,这表明控制牙周病的潜力.

科学领域:

微生物学微生物学
口腔健康口腔健康
细菌的传播细菌的传播

背景情况:

N-同类素乳 (AHLs) 是细菌的定数感应 (QS) 中的关键信号分子.
像牙斑一样的口腔生物膜涉及复杂的多种相互作用.
在口腔微生物群和牙斑形成中,AHL介导的QS的作用越来越被认可,但不太了解.

研究的目的:

调查AHL介导的QS在牙斑形成和社区结构中的作用.
探索操纵AHL信号对口腔微生物种群的影响.

主要方法:

在不同的大气条件下检测牙斑中的AHLs (5%的CO2与无氧).
干预AHL乳酶以破坏QS信号传输.
添加外部的AHLs来调节QS信号.
在浮游生物和生物膜状态下对微生物群体结构变化的分析.

主要成果:

在5%的二氧化碳下,在牙斑中检测到AHLs,但不是无氧条件;然而,无氧培养物仍然对AHLs有反应.
通过乳酸酶干扰QS信号增加了共生和先驱物种的丰富性.
添加外源性AHLs促进了晚期殖民者细菌物种的生长.

结论:

由AHL介导的QS对于牙斑块社区动态至关重要.
在牙斑块内的有氧和无氧环境中,AHL信号可能具有不同的作用.
干扰QS是一个潜在的治疗策略,用于牙周病管理.

更多相关视频

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

Published on: December 17, 2015

Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity

Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity

Published on: April 13, 2021

相关概念视频

Bacterial Signaling

Bacterial Signaling

Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...