在pyrroloiminoquinone生物合成过程中发生意想不到的转变.
Josseline Ramos Figueroa1, Lingyang Zhu1, Wilfred A van der Donk1
1Department of Chemistry and Howard Hughes Medical Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
bioRxiv : the preprint server for biology
|April 1, 2024
概括
罗胺基诺的生物合成比以前认为的要复杂得多. 这项研究揭示了氨基群是通过tRNA依赖的途径从甘氨酸,氨酸和白氨酸获得的,涉及新型酶.
科学领域:
- 生物化学 生物化学
- 自然产品生物合成 自然产品生物合成
- 分子生物学分子生物学
背景情况:
- 罗胺基诺是天然产品,具有已知的生物活性,来自酸盐.
- 它们的生物合成途径在很大程度上未被阐明,之前关于ammosamide生产的假设.
- 最初的步骤是通过PEptide Amino-acyl tRNA结合酶 (PEARL) 将托连接到支架上.
研究的目的:
- 为了阐明pyrroloiminoquinones复杂的生物合成途径.
- 为了确定pyrroloiminoquinone核心结构中原子的来源.
- 调查参与这一途径的关键酶的进化起源.
主要方法:
- 酶学试验描述了四种新型酶的功能.
- 对ammosamides的生物合成基因集群 (BGC) 的分析.
- 对酶家族进行比较分析,以推断进化关系.
主要成果:
- 发现阿莫萨米德的拟议生物合成途径不正确.
- 罗胺基诺中的氨基群是由甘氨酸,氨酸和白氨酸衍生而来的,通过tRNA依赖的机制被纳入.
- 一个FAD-依赖的甘氨酸氧化酶和一个氨酸还原酶对于的纳入至关重要.
- 珍珠和相关酶与ATP-GRASP蛋白家族具有共同的进化起源.
结论:
- 对于pyrroloiminoquinones的生物合成途径比以前提出的要复杂得多.
- 在天然产品生物合成中揭示了新的酶活性和基质特异性.
- 追溯了PEARLs和相关酶的进化起源到ATP-GRASP蛋白家族.
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