改变了底层的cis-regulatory逻辑,使得带动物的义务与自愿肌肉多核化发生了变化
Christopher J Johnson1, Zheng Zhang2,3, Haifeng Zhang3
1School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, USA.
bioRxiv : the preprint server for biology
|April 1, 2024
概括
在Myomaker (Mymk) 基因中的差异.
科学领域:
- 进化发育生物学 进化发育生物学
- 进行比较的基因组学.
- 肌肉的发展 肌肉的发展
背景情况:
- 脊椎动物和长袍动物共享Myomaker (Mymk) 基因,这对肌肉细胞融合至关重要.
- 在脊椎动物和长袍动物之间,Mymk的表达模式显著不同.
- 脊椎动物的骨肌肉是多核的,可以普遍表达Mymk,而带肌肉的Mymk则仅限于特定的肌肉类型.
研究的目的:
- 为了研究底层的Cis调节机制差异性Mymk表达在衣动物和脊椎动物.
- 确定控制不同肌肉类型Mymk基因激活的转录因子.
- 建议在衣动物和脊椎动物的最后一个共同祖先中进行Mymk调节的模型.
主要方法:
- 对Mymk推广地区的比较分析.
- 识别转录因子结合部位.
- 对cis调节元件的功能研究 (隐含).
主要成果:
- 在Mymk促进体中Cis调节序列的差异解释了不同的表达模式.
- 脊椎动物的Mymk转录完全依赖于肌源性调节因子 (MRFs).
- 形Mymk转录需要MRFs和早期B细胞因子 (Ebf) 的组合,与特定的促进体结合部位相关联.
结论:
- 在Mymk cis-regulatory元素的进化变化塑造了肌肉发育的多样性.
- 衣鱼中MRFs和Ebf的组合作用代表了与脊椎动物Mymk调节的分歧.
- 了解这些差异可以揭示神经细胞融合的祖先调节.
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