Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

High-Throughput Aptamer Characterization via Real-Time Nuclease Digestion.

Journal of the American Chemical Society·2026
Same author

Affinity Maturation of a Fentanyl Aptamer by Motif-SELEX.

JACS Au·2026
Same author

Reagentless Real-Time ATP Monitoring with New DNA Aptamers.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

High-Contrast Aptamer-Based Merocyanine Displacement Assays for Sensitive Small Molecule Detection.

ACS sensors·2025
Same author

Exploring the relationship between aptamer binding thermodynamics, affinity, and specificity.

Nucleic acids research·2025
Same author

Improving Aptamer Affinity and Determining Sequence-Activity Relationships via Motif-SELEX.

Journal of the American Chemical Society·2025

相关实验视频

Updated: Jun 29, 2025

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
08:23

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

12.2K

开发基于阿普塔默的色度测量阿片类药物测试.

Juan Canoura1, Obtin Alkhamis1, Matthew Venzke1

  • 1Department of Chemistry, North Carolina State University, 2620 Yarbrough Dr., Raleigh, North Carolina 27695, United States.

JACS Au
|April 1, 2024
PubMed
概括
此摘要是机器生成的。

基于aptamer的新传感器可以快速地在现场检测海洛因和氧化等阿片类药物. 这些色度测试可在复杂的混合物中提供准确的识别,有助于打击阿片类药物滥用.

更多相关视频

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.2K
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

9.8K

相关实验视频

Last Updated: Jun 29, 2025

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
08:23

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

12.2K
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.2K
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

9.8K

科学领域:

  • 生物技术是生物技术.
  • 分析化学 分析化学
  • 法医科学 法医科学 法医科学

背景情况:

  • 在美国,每年阿片类药物过量死亡人数超过8万.
  • 在现场快速识别被扣押的阿片类药物对于执法至关重要.
  • 目前的方法,如化学测试缺乏特异性,拉曼光谱与不纯样本作斗争.

研究的目的:

  • 开发用于检测吗啡和相关阿片类化合物的新型现场传感器.
  • 为非法阿片类药物识别创建特定和敏感的检测方法.

主要方法:

  • 在实验室中选择阿胺 (寡核酸亲和反应剂) 用于特定的阿片类药物识别.
  • 利用并行和串行以及切换选择策略来隔离阿普坦.
  • 采用高通量测序和基于外核酶的查来识别最佳的体.
  • 开发了使用高性能体的色度测量染料位移试验.

主要成果:

  • 隔离的阿普坦体识别了海洛因,吗啡,可代因,可,摩,氧可和氧摩.
  • 开发了可检测阿片类药物 (如海洛因,氧化) 的色度测定方法,其度低至0.5μM.
  • 测试证明了复杂矩阵的特异性,例如药物片和药物混合物,其性能优于马奎斯测试.
  • 在几秒钟内实现了特定阿片类药物的肉眼识别.

结论:

  • 基于aptamer的色度测试为现场阿片类药物检测提供了一种快速,具体和敏感的方法.
  • 这些传感器可以有效地在具有挑战性的现实世界样本中识别阿片类药物.
  • 开发的技术为打击阿片类药物贩运和滥用提供了有价值的工具.