新型生物活性纳米球显示出对多种内牙病原体的有效抗菌作用
Jin Liu1,2, Haoze Wu1, Jun Qiu1
1State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Department of Operative Dentistry and Endodontics, School of Stomatology, The Fourth Military Medical University, 145 West Chang-le Road, Xi'an, 710032, Shaanxi, PR China.
Heliyon
|April 1, 2024
概括
新开发的含有四级聚甲酸盐 (QAPM) 的生物活性眼镜显示出强大,持久的抗菌活性,对抗关键的内牙病原体. 这种材料还表现出极好的生物相容性,将其定位为下一代内牙密封器的有希望的替代品.
科学领域:
- 生物材料科学 生物材料科学
- 牙科材料 牙科材料
- 抗菌剂 抗菌剂 抗菌剂
背景情况:
- 内牙感染通常涉及持久性病原体,如Enterococcus faecalis.
- 目前的内密封剂在长期抗菌疗效和生物相容性方面可能存在局限性.
- 需要新的材料来改善内牙治疗的结果.
研究的目的:
- 评估含有四级聚甲酸盐 (QAPM) 的新型生物活性玻璃杯 (BGs-HAEMB) 的抗菌活性.
- 在体外和体内评估BG-HAEMB的细胞毒性和生物相容性.
- 为了比较BGs-HAEMB与临床使用的内牙密封剂.
主要方法:
- 对E. faecalis,Streptococcus sanguis和Porphyromonas endodontalis的浮游生物和生物膜形式进行了抗菌活性测试,使用亚格扩散,直接接触和活/死染色.
- 细胞毒性通过CCK-8试验进行评估.
- 使用皮肤植入模型评估生物相容性.
主要成果:
- 与Endofill,AH Plus和iRoot SP相比,BGs-HAEMB在14天和28天后对测试的病原体产生了优越的抗菌作用.
- SEM和CLSM证实了BGs-HAEMB的显著杀死微生物.
- 在BGs-HAEMB治疗组中观察到低细胞毒性 (88.6%102.9%RGR) 和最小的炎症 (I级).
结论:
- BGs-HAEMB提供了对内牙病原体的稳定和长期抗菌作用.
- 该材料在体外和体外表现出极好的生物相容性.
- BGs-HAEMB代表了开发先进内牙密封器的有希望的候选人.
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