一种潜在的益生菌酵母分离物的后生物特征,并将其微封装在被基酸盐层层地涂层的酸盐珠中
Delasa Rahimi1, Alireza Sadeghi1, Mahdi Kashaninejad1
1Department of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Heliyon
|April 1, 2024
概括
这项研究确定了Saccharomyces cerevisiae作为一种益生菌酵母,具有强大的抗氧化和抗真菌特性. 在酸盐和酸盐中封装增强了其在模拟的胃肠道条件下生存的可能性,显示了食品应用的潜力.
科学领域:
- 食品微生物学 食品微生物学
- 生物技术是生物技术.
- 益生菌和益生菌和益生菌.
背景情况:
- 益生菌 (PRO) 面临着生存和生物安全方面的挑战.
- 后生菌和封装PRO正在获得人们对其功能改善的关注.
- 研究酵母分离物对益生菌和后生菌潜力的研究至关重要.
研究的目的:
- 为了评估Saccharomyces cerevisiae分离的后生物能力.
- 评估酸盐 (Alg) 和酸盐 (Ch) 封装在模拟胃肠道 (SGI) 条件下对酵母生存的影响.
- 描述抗氧化剂,植物酸盐降解和抗奥克拉托克辛A (OTA) 活性.
主要方法:
- 使用PCR测序识别酵母分离物.
- 评估SGI条件下的生存,粘附和抗菌活性.
- 评估无细胞超眠物 (CFS) 和细胞的抗氧化,植物酸盐降解和抗OTA活性.
- 使用Alg和Ch的微封装,通过SEM,FTIR和zeta潜力进行表征.
主要成果:
- 在SGI条件下,Saccharomyces cerevisiae分离物显示出高生存率 (95.74%),具有良好的粘附性和对Listeria monocytogenes和Aspergillus niger的抑制活性.
- 酵母CFS表现出显著的抗氧化剂 (84.35%) 和植物酸盐降解 (56.19%) 活动.
- 死细胞的抗OTA活性高于活细胞;CFS没有抗OTA或抗菌作用.
- 用Alg和Ch (77.02%的疗效,直径1059微米) 进行微封装,在SGI条件下显著改善了酵母的存活率.
结论:
- 单独的Saccharomyces cerevisiae具有宝贵的后生物性质,包括抗氧化和植物酸盐降解能力.
- 使用酸盐和奇托桑进行封装有效地提高了在恶劣的胃肠道条件下益生菌酵母的存活率.
- 建议进一步对人类和工业食品进行试验,以便在实践中应用这种封装益生菌酵母.
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