揭示捷克共和国档案中的微生物多样性:对代谢活跃的空气微生物的研究

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Tereza Branysova¹, Nicole Petru¹, Marco A Lopez Marin²

¹Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Technicka 3, 166 28, Prague, Czech Republic.

Heliyon

|April 1, 2024

概括

空气中的微生物威胁着文化遗产和档案馆员工的健康. 这项研究比较了培养依赖和培养独立的方法,发现了关键物种,并通过复苏促进因子 (Rpf) 改进了细菌检测.

科学领域:

微生物学微生物学
保护科学保护科学
环境健康环境健康

背景情况:

生物退化对图书馆和档案馆的文化遗产保护构成重大威胁.
空气中的微生物是影响珍贵收藏品的生物降解剂的主要来源.
了解微生物污染对于保护遗产和确保工作场所安全至关重要.

研究的目的:

监测和比较在捷克档案中识别空气中的微生物的培养依赖和培养独立方法.
识别对文化遗产和人类健康构成风险的微生物物种.
评估不同种植媒介的有效性和复苏促进因子 (Rpf) 的作用.

主要方法:

使用各种种植媒介的培养依赖策略,包括Reasoner的2A (R2A) 和R2A + Rpf.
采用基于RNA的培养独立测序来进行全面的微生物鉴定.
比较了两种识别方法之间的重叠和差异.

主要成果:

确定了几种可能对文化遗产和档案员工造成伤害的微生物物种.
在不同档案室中展示了种植媒介的多种有效性,需要多种媒介类型.
当与R2A介质一起使用时,复苏促进因子 (Rpf) 显著增加了多达30%的细菌培养能力.

结论:

结合培养依赖和培养独立的方法,可以更全面地了解空气中的微生物群落.
积极监测和评估微生物空气污染对于防止遗产恶化至关重要.
更好地了解微生物风险可以带来更好的保存策略和更健康的档案环境.

关键词:

文化依赖的方法文化依赖的方法照明灯 MiSeqq 的时间提高培养能力提高培养能力微生物空气分析对RNA分析进行RNA分析.

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