皮肤外皮评估工具包 (PAT):用于皮肤外皮测量的一种定量和大规模选方法
Gonzalo Villarino1, Signe Dahlberg-Wright1, Ling Zhang1
1Plant Molecular and Cellular Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.
Plant phenomics (Washington, D.C.)
|April 1, 2024
概括
我们开发了PAT,这是一个用户友好的管道,使用深度学习来自动测量Arabidopsis根中的外皮长度. 这种工具准确量化了外皮的形成,有助于对这种重要的植物保护组织进行遗传研究.
科学领域:
- 植物生物学 植物生物学
- 发育生物学是发展生物学.
- 保护组织的保护组织.
背景情况:
- 周皮是植物中重要的保护组织,类似于表皮,对环境压力和病原体进行屏蔽.
- 由于其晚期形成,成熟区域局部化和不透性,对根周皮发育的研究是有限的.
- 周皮膜 (外皮) 长度是评估跨基因型和治疗方法周皮膜形成的关键指标.
研究的目的:
- 开发一个高吞吐量,用户友好的管道,用于准确的外皮长度测量Arabidopsis thaliana根.
- 通过简化外皮层的评估来促进大规模的遗传研究和选.
- 评估开发方法的可靠性和可重复性.
主要方法:
- 开发周皮层评估工具包 (PAT),集成染色,自动成像和深度学习分析.
- 应用PAT来测量20个Arabidopsis自然连接中的外皮长度.
- 自动PAT测量与人类专家测量的比较.
主要成果:
- 与专家测量相比,PAT可以准确地检测和测量Arabidopsis根中的外皮长度,效率为94%.
- 管道在各种自然连接中显示出强烈的相关性和可重复性.
- 周皮长度可以区分周皮发育突变物与野生类型,但这是一个需要仔细实验设计的塑性特征.
结论:
- PAT管道为高通量外皮膜长度量化提供了强大的解决方案,大大推进了外皮膜研究.
- PAT促进了植物保护机制的综合遗传研究和选.
- 虽然Arabidopsis的自动化显微镜有效,但对其他物种或非自动化系统可能需要适应,强调需要控制和复制.
相关概念视频
01:05Data Collection III
The physical assessment examines the patient for objective data that defines the patient's condition, and aids in formulating the nursing care plan. The purpose of physical assessment is a health status appraisal, which includes identifying health problems, and establishing a database for nursing intervention.
The principles to begin the physical assessment include conducting a comprehensive or problem-related history in a quiet, well-lit room, emphasizing privacy and comfort for the patient.
The principles to begin the physical assessment include conducting a comprehensive or problem-related history in a quiet, well-lit room, emphasizing privacy and comfort for the patient.
01:23Assessment of the Abdomen III: Palpation
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01:27Assessment of the Cardiovascular System III: Palpation
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Jugular Venous Pressure (JVP) Measurement
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