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用抗体标记的金纳米粒子通过激光切除合成,以检测SARS-CoV-2抗原尖峰

Asri Sulfianti¹, Vidhia Tiara Sopandi², Isnaeni Isnaeni³

¹Research Center for Vaccine and Drugs, National Research and Innovation Agency Republic of Indonesia (BRIN), LAPTIAB Building no 611-612, KST BJ Habibie, Serpong, Tangerang Selatan, Banten 15310, Indonesia.

|April 1, 2024

概括

用激光切除的黄金纳米粒子 (AuNPs) 与抗SARS-CoV-2抗体功能化,被开发用于快速检测. 这些AuNPs-mAb合物在横流免疫试验中成功检测到SARS-CoV-2尖端抗原.

科学领域:

纳米技术纳米技术
免疫学免疫学免疫学
生物医学工程生物医学工程

背景情况:

侧流免疫试验 (LFIA) 可以快速检测严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2).
黄金纳米粒子 (AuNPs) 对LFIA敏感性至关重要,可以使用激光切除来合成,以获得纯度和生物相容性.

研究的目的:

为了合成和描述激光切除的AuNP.
将这些AuNP与SARS-CoV-2抗尖峰单克隆抗体 (mAb) 结合起来.
评估AuNPs-mAb合物作为LFIA中用于检测SARS-CoV-2的信号记者.

主要方法:

通过激光切除合成AuNPs.
描述AuNP的大小,分布和表面潜力.
对抗尖端SARS-CoV-2 mAb与AuNPs的结合.
在半粘性LFIA中对结合物的性能进行评估.

主要成果:

激光切除的AuNPs在520nm时表现出最佳的吸收率.
AuNPs-mAb结合物在10 ng水平上成功检测到SARS-CoV-2尖端抗原.
为了确定AuNP稳定,确定了最佳的mAb固定度和缓冲条件.

结论:

与抗SARS-CoV-2 mAb功能化的激光切除AuNP作为有效的信号报告员.
这种方法可以开发敏感的LFIA用于SARS-CoV-2抗原检测.

关键词:

这就是Covid-19的原因.这是一种抗SARS-CoV-2的药物.侧流免疫测试是一种侧流免疫测试.这是一种单克隆抗体.

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