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在状细胞小鼠骨髓中与年龄相关的形态功能变化骨髓介质细胞流体细胞

Felipe A Rós^1,2, Péricles N M da Costa¹, Jonathan Milhomens¹

¹Blood Center of Ribeirão Preto - Ribeirão Preto Medical School, University of São Paulo, 2501 Tenente Catão Roxo Avenue, 14051-060, Ribeirão Preto, São Paulo, Brazil.

Hematology/oncology and stem cell therapy

|April 1, 2024

概括

年龄影响状细胞病 (SCD) 小鼠骨髓中介质 stromal 细胞 (BM-MSCs) 的基因表达. 年轻的SCD BM-MSCs支持造血干细胞 (HSC) 维护,而较老的细胞表现出促炎变化.

科学领域:

血液学血液学血液学
干细胞生物学干细胞生物学
细胞生物学细胞生物学

背景情况:

骨髓介质干细胞 (BM-MSCs) 对于造血干细胞 (HSC) 利基至关重要.
状细胞疾病 (SCD) 影响HSC功能,但其对BM-MSCs的影响尚未完全理解.
这项研究调查了SCD小鼠的BM-MSC与年龄相关的变化.

研究的目的:

探索状细胞病 (SCD) 的小鼠的BM-MSCs的变化.
在SCD的背景下,确定年龄和BM-MSC基因表达之间的关系.
评估SCD对BM-MSC功能与HSC维护和炎症相关的影响.

主要方法:

从Townes-SS (SCD) 小鼠和年龄匹配的对照 (Townes-AA和C57BL/6 J) 中分离出了BM-MSC.
免疫表型分析证实了MSC特征.
进行了体外分化试验.
基因表达分析的重点是HSC维护和炎症标志物.

主要成果:

来自Towns-SS小鼠的BM-MSCs表现出典型的MSC免疫表型和差异化能力.
年轻的 (30天) SCD BM-MSCs 显示HSC支持基因 (Cxcl12,Vegfa,Angpt1) 的表达增加和炎症基因 (Tnfa,Il-6) 的减少.
较老 (60天) 的SCD BM-MSCs显示HSC维护基因表达减少和增强的促炎性基因表达.

结论:

在SCD背景下,年龄是BM-MSC基因表达的显著改变因素.
SCD BM-MSC 呈现年龄相关的功能转变,影响 HSC 支持和炎症.
这些发现凸显了SCD中BM-MSC利基的动态性质.

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