毒素暴露后血清前列腺素不依赖于外部毛细胞损失
Megan S Harrison, Brittany G Driscoll, Jason Farnsworth
1Department of Surgery, Division of Otolaryngology-Head & Neck Surgery, University of Connecticut School of Medicine, Farmington, Connecticut.
概括
环极素 (CDX) 导致血清前列素的增加,独立于外部毛细胞 (OHC) 的损失. 这种N-glycosylated prestin burst可以作为干预的早期耳毒性生物标志物.
科学领域:
- 耳毒性研究研究
- 发现生物标志物的发现.
- 听觉神经科学 听觉神经科学
背景情况:
- 血清前列素是潜在的耳毒性生物标志物.
- 之前的研究引入了一个循环德克斯 (CDX) 耳毒性模型.
- 在这个模型中,需要进一步描述血清前列蛋白的特征.
研究的目的:
- 为了研究CDX诱导的血清前列蛋白变化和外发细胞 (OHC) 损失之间的关系.
- 为了描述血清前列素爆发的性质.
- 评估血清前列素作为耳毒性早期生物标志物.
主要方法:
- 几内亚猪接受了不同剂量的CDX (750,3,000,4,000毫克/公斤).
- 在15周内,使用自动化西方分析量化血清前列素水平.
- 进行毛毛细胞计数以评估OHC损伤.
主要成果:
- 所有的CDX剂量都诱导了N-糖基化~134-kDa的前列腺突发.
- 只有在较高的CDX剂量 (3,000和4,000毫克/公斤) 时才观察到显著的OHC损失.
- 血清前列蛋白恢复到基线水平,即使有OHC损失,也保持稳定.
结论:
- CDX诱导的~134-kDa的前列蛋白爆发是一种后翻译修饰的,N-糖化形式的前列蛋白.
- 血清前列素是耳毒性的一个有前途的生物标志物,因为它的升高并不取决于OHC损失.
- 这一发现允许早期检测和干预耳毒性.
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