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编码分类酶的基因,srtA和srtC,介导着Enterococcus faecalis的OG1RF在Helicoverpa zea胃肠道中的持久性

Jerreme J Jackson¹, Samantha Heyer¹, Geneva Bell¹

¹Department of Biology, University of Northern Iowa, Cedar Falls, IA, United States.

Frontiers in microbiology

|April 2, 2024

概括

排序酶对于Enterococcus faecalis在昆虫胃肠道中的持久性至关重要. 缺乏类酶A或C的突变体在Helicoverpa zea模型中表现出减少生物膜形成和受损的持久性.

科学领域:

微生物学微生物学
昆虫病理学昆虫病理学
细菌病原体的产生

背景情况:

菌 (Enterococcus faecalis) 是一种常见的肠道细菌和机会性病原体.
了解胃肠道 (GIT) 中的细菌持久机制至关重要.
无脊椎动物模型为宿主微生物相互作用提供了独特的见解.

研究的目的:

为了研究分类酶在E. faecalis在昆虫GIT.内持久性的作用.
描述与无脊椎动物宿主结合的细菌机制.
用一种非破坏性昆虫模型来研究GIT细菌的持久性.

主要方法:

开发了一种非破坏性采样模型,使用Helicoverpa zea幼虫.
创建了同源的E. faecalis OG1RF分类酶突变 (ΔsrtA, ΔsrtC).
在H. zea GIT模型中评估了细菌生长,体外生物膜形成和持久性.

主要成果:

与野生型E. faecalis.相比,Sortase突变体表现出类似的浮游生物生长,但与野生型E. faecalis.相比,生物膜形成受损.
在H. zea GIT模型中,ΔsrtA和ΔsrtC突变均表现出显著降低的持久性.
这项研究突出了索尔塔酶在E. faecalis GIT持久性中的作用.

结论:

排序酶对于E. faecalis在昆虫GIT中的持久性和生物膜形成至关重要.
赫利科维尔巴 (Helicoverpa zea) 模型为研究白虫中细菌GIT持久性的研究提供了一个有价值的工具.
这项研究揭示了微生物与无脊椎动物宿主的新型分子机制.

关键词:

菌 (Enterococcus faecalis) 是一种菌.这是一架直升机的飞行器.生物膜是一种生物膜.持续性持久性排序排序排序排序

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