14-3-3ζ通过调节脂肪细胞分化的早期阶段的染色质可访问性来调节脂肪生成
S A Rial1,2, Z You3, A Vivoli1,2
1Department of Medicine, Université de Montréal, Montreal, QC, Canada.
bioRxiv : the preprint server for biology
|April 2, 2024
概括
14-3-3ζ对于脂肪前体细胞以性别依赖的方式分化至关重要. 这种支架蛋白作为表观遗传调节剂,影响脂肪细胞发育期间的染色质可访问性和脂肪基因表达.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 代谢过程中的代谢.
背景情况:
- 众所周知,支架蛋白14-3-3ζ调节脂肪生成和脂肪性.
- 通过14-3-3ζ发挥其功能的精确时间和细胞类型 (成熟脂肪细胞与脂肪前体细胞) 尚不清楚.
研究的目的:
- 要确定14-3-3ζ是否调节成熟脂肪细胞或脂肪前体细胞 (APC).
- 研究14-3-3ζ在APC分化过程中的基转录程序和表观遗传调节中的作用.
主要方法:
- 产生了14-3-3ζ淘汰赛小鼠模型 (Adipoq-14-3-3ζKO和Pdgfra-14-3-3ζKO).
- 通过CRISPR-Cas9基因编辑,创建TAP标记的14-3-3ζ表达3T3-L1前脂质细胞,用于核互动原子分析.
- 使用ATAC-seq.进行染色体可访问性分析.
主要成果:
- 14-3-3ζ在APC分化中起着取决于性别的作用,在雄性和雌性小鼠中影响体重和脂肪质量不同.
- 核互动原子分析显示,染色体重塑调节器 (DNMT1,HDAC1) 富含14-3-3ζ.
- 14-3-3ζ 枯竭改变了关键基因基因位点上的染色质可访问性,并与基因基因的改变表达相关.
结论:
- 14-3-3ζ是脂肪生成的重要表观遗传调节剂,主要作用于脂肪前体细胞.
- 14-3-3ζ的核相互作用体可以识别参与脂肪生成的新型调节者和途径.
- 了解14-3-3ζ的作用,可以了解脂肪发育的性别特异性机制以及潜在的治疗点.
关键词:
在14-3-3ζζ中.ATAC-seqq 的使用情况.脂肪组织的脂肪组织.脂肪细胞的原始细胞细胞.脂质生成 (adipogenesis) 是一种不同化的差异化差异化.脚手架蛋白质是一种脚手架蛋白质.更多相关视频
06:08Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
Published on: May 19, 2023
2.1K
11:11Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
Published on: March 17, 2023
2.3K
相关概念视频
Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
Chromatin Modification in iPS Cells
1.6K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.6K
Epigenetic Regulation
3.0K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
X-chromosome...
3.0K
Co-activators and Co-repressors
7.3K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.3K
Chromatin Structure Regulates pre-mRNA Processing
7.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
7.0K
Inheritance of Chromatin Structures
6.2K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.2K
