青少年的甲状腺皮层抑制改变了前额外刺激-抑制平衡
David Petersen1,2, Ricardo Raudales1,2, Ariadna Kim Silva1,2
1Department of Psychiatry, Columbia University Irving Medical Center, New York, NY 10032, USA.
bioRxiv : the preprint server for biology
|April 2, 2024
概括
在青春期抑制前额叶皮质的thalamic输入会损害认知功能. 这项研究揭示了突触连接的特定变化,突出显示了脑皮层与甲状腺皮层通路对大脑发育的重要性.
科学领域:
- 神经科学是一个神经科学.
- 发育神经科学的发展神经科学.
- 认知神经科学 认知神经科学
背景情况:
- 青少年前额叶皮层 (PFC) 的发展对于成人认知至关重要.
- 乳头皮层投射在PFC成熟中发挥作用,但它们对青春期电路连接的具体影响尚未完全理解.
研究的目的:
- 为了研究在特定的青少年窗口 (产后20-35天) 中抑制thalamo-prefrontal投射如何影响PFC金字塔神经元的突触输入.
- 确定这种抑制对PFC电路平衡和成人认知功能的后果.
主要方法:
- 利用化学遗传工具,从出生后20至35天的小鼠中选择性抑制thalamo-prefrontal投射.
- 通过切片生理学在抑制后24小时评估不同层和投射目标的PFC金字塔神经元中的突触电流 (刺激和抑制).
- 在接受青少年抑制期的成年小鼠中评估认知功能.
主要成果:
- 抑制导致II/III层NAc投射神经元和V/VI层中脊柱 thalamus投射神经元的刺激和抑制电流频率下降.
- 层V/VINAc投射神经元显示激发和抑制电流幅度增加.
- 反侧的mPFC投射神经元表现出增强的抑制电流频率,总体激发-抑制平衡仅在这些状投射神经元中发生变化.
- 青少年抑制导致成年小鼠的认知缺陷.
结论:
- 青春期的thalamo-prefrontal通路抑制改变了特定的PFC神经元群体中的突触可塑性.
- 兴奋抑制平衡的恒温调节似乎发生在皮层下,而不是皮层内途径.
- 在青春期针对性地抑制前额内连接可能对正常的PFC电路成熟和认知功能至关重要.
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