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乙菌抗原B对巨细胞炎症激活的调节作用

Ana Maite Folle^1,2, Sofía Lagos Magallanes^1,2, Martín Fló³

¹Unidad de Inmunología, Instituto de Química Biológica, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.

Frontiers in cellular and infection microbiology

|April 2, 2024

概括

原生和重组的 Echinococcus granulosus抗原B (EgAgB) 调节巨细胞的炎症反应. 这项研究揭示了EgAgB8/1亚单元.

科学领域:

免疫学免疫学免疫学
寄生虫学的寄生虫学
分子生物学分子生物学

背景情况:

鸟利用脂质结合蛋白来运输营养,其中埃奇诺科克细粒菌抗原B (EgAgB) 是一个关键的脂蛋白.
EgAgB对于囊性赤道球菌病的诊断至关重要,但其免疫生物学的作用尚不清楚,特别是在原生与变质形式之间.
之前关于EgAgB免疫调节特性的研究使用了变质制剂,限制了对原生EgAgB效应的解释.

研究的目的:

分析原生EgAgB (nEgAgB) 和重组EgAgB8/1亚单元 (rEgAgB8/1) 在巨细胞上的免疫调节作用.
为了比较nEgAgB和rEgAgB8/1对体外和体外炎症反应的影响.
阐明EgAgB8/1亚单元对EgAgB免疫调节功能的贡献.

主要方法:

使用新型抗EgAgB8/1纳米体的免疫亲和染色学净化nEgAgB和rEgAgB8 / 1.
两种制剂的脂蛋白大小和脂质组成的表征.
使用人类和小鼠巨细胞进行体外分析,以评估LPS驱动的激活,细胞因子分泌 (IL-1β,IL-6,IL-12p40,IFN-β) 和氧化 (NO) 生成.
在体内研究评估LPS诱导的细胞因子产生 (IL-6,IL-10) 和巨细胞激活标志物 (MHC-II,CD86,CD40) 在腹膜.

主要成果:

nEgAgB和rEgAgB8/1表现出不同的尺寸和脂质配置文件,rEgAgB8/1形成较大的脂蛋白,脂质较少多样化.
在体外,nEgAgB和rEgAgB8/1都抑制了LPS诱导的巨细胞激活,减少了细胞因子的分泌和NO的产生.
在体内,这两种药物都调节了LPS诱导的细胞因子产生和巨细胞激活,尽管rEgAgB8/1的效果不那么强大.

结论:

已经证实EgAgB是Echinococcus granulosus的一个免疫调节成分.
EgAgB8/1亚单元在EgAgB调节巨细胞炎症激活方面发挥着重要作用.
虽然nEgAgB的脂质成分可能有所贡献,但EgAgB8/1亚单元对巨细胞调节的直接作用得到了支持.

关键词:

埃奇诺科库斯颗粒状的高LBP家族的家庭.抗原B是一种抗原.这里是Cestodes的Cestodes.免疫调节免疫调节免疫调节蛋白质脂质蛋白质 (lipoprotein) 是一种脂质蛋白质.巨细胞是一个巨细胞.

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