相关实验视频

Updated: Jun 29, 2025

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Published on: July 8, 2021

对正常和退化的人类椎间盘进行并列质量基于标签的蛋白质组分析

Yang Fu¹, Xiao-Qin Huang¹, Hang-Bo Qu¹

¹Department of Orthopedics, Zhejiang Hospital, Hangzhou, Zhejiang Province, People's Republic of China.

Journal of pain research

|April 2, 2024

概括

椎间盘退化 (IVDD) 涉及蛋白质水平的改变,其中CYCS,RAC1和PSMD14被确定为关键参与者. 相关途径包括病毒感染和癌症,为腰部疼痛 (LBP) 提供了新的见解.

科学领域:

生物化学生物化学
分子生物学分子生物学
蛋白质组学是指蛋白质组学.

背景情况:

椎间盘退化 (IVDD) 是腰部疼痛 (LBP) 的主要原因之一.
导致IVDD的精确分子机制和调节途径在很大程度上是未知的.
了解这些因素对于开发有效的LBP治疗至关重要.

研究的目的:

在退化椎间盘样本中识别差异丰富的蛋白质.
阐明IVDD所涉及的分子途径和潜在机制.
为了发现治疗腰部疼痛的新治疗点.

主要方法:

使用MRI对Pfirrmann II级和IV级IVDD核脉样本的比较分析.
使用液体染色学-并联质谱法 (LC-MS/MS) 进行定量蛋白质组分析.
生物信息分析包括基因和基因组的京都百科全书 (KEGG) 和基因本体学 (GO) 丰富,以及西方斑点验证.

主要成果:

在两组IVDD之间确定了70种不同丰富的蛋白质 (30增加,40减少).
突出显示了CYCS,RAC1和PSMD14作为可能在IVDD中发挥重要作用的蛋白质.
与包括爱斯坦-巴尔病毒感染,病毒性心肌炎,结直肠癌,非酒精性脂肪肝疾病 (NAFLD) 和肌缩性侧面硬化症 (ALS) 在内的途径相关的IVDD.

结论:

CYCS,RAC1和PSMD14被认为是IVDD病变发生的关键蛋白质.
特定的病毒感染和疾病,如结肠直肠癌,NAFLD和ALS与IVDD共享共同的途径.
这些发现为进一步研究LBP的分子基础和潜在的治疗干预提供了基础.

关键词:

在 LC―MS/MS 中.TMTT TMTT 是一个很好的方法.腰部疼痛腰部疼痛腰部疼痛腰部疼痛它的核是脉冲的脉冲.蛋白质组学蛋白质组学

更多相关视频

A Proinflammatory, Degenerative Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease.

A Proinflammatory, Degenerative Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease.

Published on: February 14, 2021

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...