在工程 Corynebacterium glutamicum 中生物合成阿皮基因糖化物
Obed Jackson Amoah1, Samir Bahadur Thapa1, Su Yeong Ma1
1Department of Life Science and Biochemical Engineering, Sun Moon University, Asan-si 31460, Republic of Korea.
Journal of microbiology and biotechnology
|April 2, 2024
概括
这项研究开发了一种具有成本效益的微生物系统,用于使用工程*C. glutamicum*进行黄类糖化. 改造的细菌有效地产生了高产的阿皮基宁糖化物,改善了它们对潜在的大规模应用的性能.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 黄酸葡萄糖基化增强了溶解性和生物活性,使黄酸葡萄糖成为生物合成的有价值标.
- 目前用于大规模的黄类糖化酶的酶方法受到低产品产量和昂贵的UDP糖的限制.
研究的目的:
- 设计一个*C. glutamicum*细胞工厂,以高效,低成本,大规模生物合成黄类葡萄糖类.
- 为了优化使用代谢工程细菌系统的apigenin葡萄糖酸的生产.
主要方法:
- 在C. glutamicum*中,来自*Bacillus licheniformis*的一种乱交的葡萄糖转移酶 (YdhE) 的异质表达.
- 在C. glutamicum*中,UDP-葡萄糖合成基因 (*galU1*和 *pgm*) 的内源性过度表达.
- 优化葡萄糖化条件,包括温度,用于原蛋白葡萄糖化.
主要成果:
- 工程*C. glutamicum*有效地产生了各种类型的原生蛋白糖化物.
- 获得了高产量的阿比基宁糖化物,包括在25°C时4.2mM的阿比基宁-4'-O-β-糖化物.
- 在40小时后,在37°C时观察到显著的二糖化物 (APG3和APG4) 生产.
结论:
- 开发的 *C. glutamicum* 细胞工厂系统使得无需昂贵的 UDP 糖,便于成本效益的,大规模的黄类糖化.
- 该平台可用于修改各种植物二次代谢物,以增强它们的药理动力学活性.
- 该工程系统为生物活性黄类衍生物的工业生产提供了一个有前途的方法.
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