选植物促进生长的细菌具有抗菌性质的高原大米
Khammool Khamsuk1, Bernard Dell2, Wasu Pathom-Aree3
1Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand.
Journal of microbiology and biotechnology
|April 2, 2024
概括
来自大米根的有益细菌显示植物生长促进和疾病控制. 巴西勒斯种类 (Bacillus sp.) 的存在 KK281显示出增强高地大米生长和管理疾病的巨大潜力.
科学领域:
- 农业微生物学 农业微生物学
- 植物病理学 植物病理学
- 生物技术是生物技术.
背景情况:
- 米种植面临着病原体和低于最佳生长条件的挑战.
- 大米树根球中的有益细菌可以增强植物的健康和生产力.
- 识别具有促进植物生长和抗微生物特性的新型细菌菌株对于可持续农业至关重要.
研究的目的:
- 从大米根和树根球中分离和描述有益细菌.
- 评估它们的促进植物生长的特性,包括酸盐溶解和英多尔-3-酸 (IAA) 生产.
- 评估它们对关键米病原体的抗微生物活性,并确定进一步开发的有希望的候选物.
主要方法:
- 从水根球中分离和选315种细菌菌株.
- 对促进植物生长的特征的测定:酸盐溶解和IAA生产.
- 对Curvularia lunata,Bipolaris oryzae和Xanthomonas oryzae pv.进行抗菌活性测试. 采用双培养和无细胞超的方法.
- 高级菌株Bacillus sp.的识别和表征 KK281,包括脂分析 (LC-ESI-MS/MS) 和细胞毒性测试.
主要成果:
- 几个分离物表现出促进植物生长的特征;KK074产生高IAA (362.6微克/毫升) 和KK007溶解酸盐 (714.2微克/毫升).
- 隔离品KK024和KK281显示出强烈的抑制对C. 卢纳塔,KK269对比B. 一个小鸟. oryzae
- 细菌 sp. 的种类. KK281表现出显著的抗微生物活性,包括3.1 mg/ml的最小抑制度 (MIC) 对X. 奥里泽 pv. 在这里. 丽泽,并产生了表面因子.
结论:
- 细菌 sp. 的种类. KK281具有显著的体外植物生长促进和疾病控制能力.
- 已识别的脂,表面素,有助于Bacillus sp. 的抗菌疗效. KK281.KKK281.KKK281.KKK281.KKK281.KKKK281.KKK281.KKKK281.KKKK281.KKKK281.KKK281.KKKK281.KKK281.KKK281.KKKK281.KKK281.KKKK281.KKK281.KKK281.KKK281.KKK281.
- 细菌 sp. 的种类. KK281 是一个有希望的候选人,用于实地试验,以增强高地大米的生长和可持续地管理疾病.
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