改善了新型酸性植物酶的热稳定性
Byung Sam Son1, So Hyeong Kim1, Hye-Young Sagong1
1Institute of Biotechnology, CJ CheilJedang Co., Suwon 16495, Republic of Korea.
Journal of microbiology and biotechnology
|April 2, 2024
概括
来自Turicimonas muris的新型植物酶TmPhy增强了动物营养并减少了污染. 突变动物在料应用中显著改善了耐热性.
科学领域:
- 生物化学 生化学
- 酶学 是一种酶学.
- 动物营养 动物营养
背景情况:
- 植物酶通过化植物酸盐来改善动物料中的营养物质可用性.
- 植物酶还可以减轻环境污染.
- 来自Turicimonas muris的新型植物酶TmPhy提供了独特的结构和功能特性.
研究的目的:
- 来自Turicimonas muris的新型植物酶TmPhy的特征.
- 提高TmPhy的热稳定性,以便在动物料中实际应用.
主要方法:
- 在Pichia系统和净化中TmPhy的基因表达.
- 确定最佳活动条件 (pH和温度).
- 蛋白质工程技术包括随机突变,二硫化键引入和N端突变以增强热稳定性.
主要成果:
- 在70°C时,TmPhy表现出双重最佳pH值 (3和6.8) 和最高活性.
- 工程突变体显示出显著增强的耐热性.
- 在80°C处理后,tmPhyMD2保持了74.1%的活性,这表明热稳定性得到了显著改善.
结论:
- TmPhy是一种有前途的植物酶,有可能提高动物料效率和环境可持续性.
- 工程TmPhy变种表现出优越的热稳定性,使它们适合料加工条件.
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