开发和实施一个集成的临床前动脉样硬化数据库
Rachel R Xiang1, Yihua Wang1, Megan M Shuey2
1Molecular Cardiology Research Institute, Tufts Medical Center, Boston, MA (R.R.X., Y.W., B.V.C., I.Z.J.).
Circulation. Genomic and precision medicine
|April 2, 2024
概括
从动脉样硬化小鼠模型中整合临床前数据可以改善基础科学发现的转化到临床护理. 这项研究创建了动脉样硬化研究的数据库,以实现全面分析和加速疾病研究.
科学领域:
- 心血管研究研究心血管研究
- 翻译医学是一种翻译医学.
- 生物信息学是一种生物信息学.
背景情况:
- 临床前动物模型对于理解人类疾病机制至关重要,但对临床实践的转化仍然有限.
- 成千上万的出版物支持动物研究的因果推断,但缺乏整合阻碍了进步.
- 对临床前数据的综合方法为改善医学中翻译成功提供了潜力.
研究的目的:
- 利用常见的小鼠模型开发一个全面的临床前动脉样硬化研究数据库.
- 促进对实验条件,干预措施和已发表研究成果的综合分析.
- 为调查动脉样硬化研究的全球影响和改进翻译结果提供资源.
主要方法:
- 从关键期刊上发表的动脉样硬化 (ApoE-knockout和LDLR-knockout) 的小鼠模型中提取的数据.
- 收集了预定义的变量,包括动物的性别,饮食,干预和斑块病理 (大小,炎症,脂质含量).
- 开发了数据标准化,干预归因和转换的程序,用于转录学软件.
主要成果:
- 创建了一个临床前动脉样硬化病数据库,整合了数百个体内测试的基因.
- 数据库包括关于实验条件和已发表的动脉样硬化研究结果的详细信息.
- 为社区使用提供了标准化的数据提取和转换方法.
结论:
- 该数据库作为一个资源,用于查询与斑块病理,细胞类型或性别相关的特定数据子集.
- 提供的方法和软件可以扩展数据集,并应用于在临床前模型中研究的其他人类疾病.
- 这种综合方法旨在提高临床前发现的转化为临床进展.
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