一种基于自我组装和细胞迁移的高密度3D管状结构屏障形成膜的制造
Seyedaydin Jalali1, Ponnambalam Ravi Selvaganapathy1,2
1School of Biomedical Engineering, McMaster University, Hamilton, ON, Canada.
Lab on a chip
|April 2, 2024
概括
研究人员开发了一种新的成型技术,以创建可 perfusable 三维 (3D) 组织屏障模型. 这种方法可以制造高细胞密度结构,模仿体内内皮质壁垒,具有前所未有的低透性.
科学领域:
- 生物材料工程 生物材料工程
- 组织工程是组织工程.
- 细胞生物学 细胞生物学
背景情况:
- 与2D模型相比,三维 (3D) in vitro模型提供了优越的生理模拟,使复杂的细胞-细胞和细胞-细胞外矩阵相互作用成为可能.
- 长时间开发具有密度,多细胞结构的可 perfusable 3D 组织屏障模型仍然是一个重大的技术挑战.
研究的目的:
- 描述一种基于成型的新方法,用于制造独立的,可 perfusable,高细胞密度的3D组织结构.
- 创建功能性组织屏障,模仿体内条件,用于药物查和疾病建模中的应用.
主要方法:
- 一种采用聚四乙烯 (PTFE) 涂层线作为模板的成型技术,在聚二甲基 (PDMS) 井内创建可 perfusable 通道.
- 含有细胞和原的生物墨水在线周围自组装,形成高细胞密度层.
- 组装后的细胞迁移,例如人静脉内皮细胞 (HUVECs),通过附着结形成功能障碍.
主要成果:
- 该方法成功地制造了具有高细胞密度的可扩展,独立的,可 perfusable 3D 组织结构.
- 通过透性测试和光成像证实,通过粘附接口构建了表现出的功能性内皮壁垒.
- 这些3D模型显示了在体外模型中报告的最低的透率,在体内内皮质屏障功能中非常接近.
结论:
- 描述的基于成型的方法为制造复杂的可 perfusable 3D 组织结构提供了一个简单且可扩展的替代方案.
- 这种技术有效地产生功能性组织障碍,适用于各种应用,包括组织工程,药物查和疾病建模.
- 开发的结构代表了体外建模的重大进步,提供了对生理条件的改进模拟.
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