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Updated: Jun 29, 2025

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
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由TaqMan多重复实时PCR开发和应用四种食源性病原体

Yinlei Xue1, Shengfang He2, Meng Li3

  • 1Food Inspection and Testing Center of Yexian County, Yexian County, Henan, China.

Foodborne pathogens and disease
|April 2, 2024
PubMed
概括

一种新的TaqMan多重实时PCR (mRT-PCR) 方法可以在食品样本中准确检测多种由食物传播的病原体,包括沙门氏菌和大肠杆菌O157. 这种快速检测为食品安全诊断中的病原体检测提供了可靠的解决方案.

关键词:
埃舍里奇亚大肠杆菌O157L.单细胞原体.黄金色的S. 黄金色的沙门氏菌spp.p.p. 的情况.塔克曼探测器探测器多重实时PCR复杂化

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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