从人体血清或血中获得N结合糖的丰富方法:一个小小的回顾
Xuyuan Chao1, Baoying Zhang1, Shengjie Yang1
1Phase I Clinical Trial Center, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038, People's Republic of China.
Carbohydrate research
|April 2, 2024
概括
人体血清和血中的N-糖化变化可能表明疾病. 本综述详细介绍了丰富N-甘氨酸和N-甘的方法,这些方法对于生物标志物发现和糖蛋白质组表征至关重要.
科学领域:
- 生物化学 生化学
- 蛋白质组学是指蛋白质组学.
- 分析化学 分析化学
背景情况:
- 人类疾病通常与血清或血中蛋白质糖化模式的改变有关.
- N-甘化是一种关键的翻译后修饰,有可能作为疾病预后和诊断的生物标志物.
- 丰富的非糖蛋白,如白蛋白干扰检测低丰富度的糖蛋白及其糖在复杂的生物样本.
研究的目的:
- 审查和阐明N-甘氨酸和N-甘氨酸丰富方法的原理.
- 突出各种富化技术的应用,实用性和局限性,用于糖蛋白质组的表征.
- 为了应对检测微异质性和人体血清和血中低糖丰度的挑战.
主要方法:
- 基于甘氨酸亲和力的丰富策略.
- 基于化学性质的分离技术.
- 静态相化学合和生物对角方法.
- 基于质谱的糖蛋白组学.
主要成果:
- 为了克服大量的血蛋白质所带来的挑战,已经开发出各种丰富方法.
- 这些方法有助于检测和表征N-glycans和N-glycopeptides.
- 成功丰富对于识别潜在的疾病生物标志物至关重要.
结论:
- 有效丰富N-甘氨酸和N-甘氨酸对于在疾病研究中推进糖蛋白组学是必不可少的.
- 了解丰富技术的原理和局限性是准确的糖蛋白质组表征的关键.
- 本综述提供了这些方法对生物标志物发现和疾病诊断的有用性的见解.
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