从Bacillus subtilis PR-C18中获取的益生菌Levon类型的果实素作为一种强大的抗菌膜剂:结构阐明和in silico分析
Juanit Thomas1, Payel Roy1, Arabinda Ghosh2
1Department of Bioengineering, NIT Agartala, Tripura, 799046, India.
Carbohydrate research
|April 2, 2024
概括
研究人员发现了一种来自 Bacillus subtilis PR-C18 的新型外聚糖化物 (EPS),具有益生菌和抗生素特性. 这种EPS显示出作为食品和制药中的功能成分的潜力.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 食品科学 食品科学 食品科学
背景情况:
- 对治疗益生菌的日益增长的需求推动了对新型外聚糖的搜索.
- 病原生物和医疗保健相关的病原体需要新的增长抑制策略.
研究的目的:
- 隔离和描述一种具有前生物和抗菌膜活性的新型外聚糖 (EPS).
- 评估EPS PR-C18作为食品和制药应用中的功能成分的潜力.
主要方法:
- 从片中分离和识别Bacillus subtilis PR-C18的细菌.
- 使用紫外线图,染色图,FTIR,NMR,SEM,风湿学,DSC-TGA和细胞毒性试验对外聚糖 (EPS) PR-C18的表征.
- 使用分子对接,模拟,MM-GBSA和PCA研究进行抗菌膜活性的in silico验证.
主要成果:
- 细菌亚菌PR-C18产生的外聚糖化物 (EPS) PR-C18具有前生物和抗生物膜活性.
- EPS PR-C18是一种分子量为3.40 × 10^4 Da的同聚合物,具有微孔结构,剪切稀释行为和出色的功能性质 (乳化,花,保留水分,抗氧化活性).
- EPS PR-C18表现出高温稳定性,生物相容性和强大的与PelD的结合亲和力,这是Pseudomonas aeruginosa的毒性因子,表明了显著的抗菌膜潜力.
结论:
- 从 Bacillus subtilis PR-C18 中衍生出来的 EPS PR-C18 具有显著的前生物和抗生物膜特性.
- EPS PR-C18的功能性和生物相容性支持其在食品和制药行业作为添加剂或辅助剂的使用.
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