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男性LUSC患者的miRNA模式 - 三向镜子:组织,血和外体

Cecilia Bica¹, Ancuta Jurj², Antonia Harangus³

¹Research Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 400337, Romania; Research Center for Advanced Medicine-MedFUTURE, Department of Translational Medicine, "Iuliu Haţieganu" University of Medicine and Pharmacy, 400347 Cluj-Napoca, Romania.

Translational oncology

|April 2, 2024

概括

三种特定的microRNAs (miRNAs) 在男性患者的肺状细胞癌 (LUSC) 组织和血中显示出改变的表达. 这些miRNAs显示为男性早期LUSC诊断的生物标志物具有前途.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物标志物发现发现

背景情况:

肺癌,特别是肺状细胞癌 (LUSC),是全球主要的健康问题.
早期诊断对于改善LUSC患者的治疗结果至关重要.
确定用于LUSC诊断的新生物标志物是一个正在进行的研究优先事项.

研究的目的:

评估男性患者LUSC组织和血中miR-21-5p,miR-155-5p和miR-181a-5p的表达水平.
为了确定这些microRNA是否可以作为男性LUSC的诊断生物标志物.
为了评估这些miRNA面板的诊断准确性,用于LUSC检测.

主要方法:

从40名男性LUSC患者的匹配瘤和相邻正常组织中进行微RNA表达分析.
使用TCGA LUSC数据集 (279个男性样本) 验证miRNA表达.
在血和来自血的细胞外囊泡 (EVs) 中分析miRNA水平.
接收器操作特征 (ROC) 曲线分析以评估诊断准确性.

主要成果:

在LUSC瘤组织中,miR-21-5p和miR-155-5p表达过高,而miR-181a-5p在LUSC瘤组织中表达过低,与正常组织相比.
这些miRNA表达变化在LUSC男性患者的血和血EV中是一致的.
三种miRNA的组合表明,在男性受试者中,LUSC的高诊断准确率高达88%.

结论:

评估的微RNAs (miR-21-5p,miR-155-5p和miR-181a-5p) 在LUSC中表现出不同的表达模式.
这些miRNA可以在血和血EV中检测到,这表明它们作为非侵入性生物标志物的潜力.
这三种miRNA的组合面板对男性群体中LUSC的早期检测具有显著的前景.

关键词:

生物标志物生物标志物细胞外囊泡中的细胞外囊泡.肺癌是一种肺癌.微RNAs 是一个微型RNA.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...