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Updated: May 10, 2026

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
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双管家规范的CRISPR-Cas12a传感平台用于ApoE单核酸多态形态基因定型

Yuedong Zhu1, Yanan Lin1, Bin Gong1

  • 1School of Pharmacy, Nantong University, Nantong, Jiangsu, 226001, China.

Biosensors & bioelectronics
|April 2, 2024
PubMed
概括

这项研究引入了一种新的Cas12a基因型识别平台,用于准确检测单核酸多态 (SNP). 该方法在从人类样本中识别ApoE基因SNP时达到100%的准确性,使潜在的现场诊断成为可能.

关键词:
在 ApoEEE 中.这就是Cas12a.基因型定制是指基因型定制.单个核酸的多态性.

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相关概念视频

Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...