了解您的测定使用EQA数据与血清肌素作为一个例子的局限性
Rachel Marrington1, Finlay MacKenzie1
1Birmingham Quality (UK NEQAS), 1732 University Hospitals Birmingham NHS Foundation Trust , Birmingham, UK.
Clinical chemistry and laboratory medicine
|April 2, 2024
概括
测试的特异性和不精确性会影响外部质量评估 (EQA) 的结果. 聚合的血清EQA材料可能会掩盖实验室测定偏差,使得难以识别分析问题. 来自罗氏科巴斯和西门子Atellica的酶类肌胺测定显示出最小的偏差.
科学领域:
- 临床化学 临床化学
- 实验室医学 实验室医学
- 分析毒理学分析毒理学
背景情况:
- 测试的特异性和不精确性对于实验室质量评估至关重要.
- 外部质量评估 (EQA) 材料需要仔细考虑其组成.
- 血清矩阵组成可以影响EQA结果和测试性能评估.
研究的目的:
- 调查血清矩阵组成 (单个与聚合的捐赠者) 对EQA结果的影响.
- 为了比较多种分析方法的测试性能,使用肌素作为模型分析剂.
- 评估血清源对EQA测试偏差和可变性的影响.
主要方法:
- 在12个月内,分析了来自16个血清矩阵的36个样本 (单个和聚合捐赠).
- 包括未经处理和增肌素的血清.
- 用各种方法评估来自英国大约500家实验室的肌素分析数据.
主要成果:
- 只有罗氏科巴斯和西门子阿特利卡的酶性肌氨酸测定在所有标本中达到5.6%的偏差目标.
- 聚合的血清材料在不同的分析方法中表现出较少的偏差变化.
- 单一供体血清显示出偏差的更大变化,取决于测试方法.
结论:
- 实验室必须了解测试限制,以避免误解EQA/IQC数据.
- 从聚合捐赠中制成的EQA材料可以稀释干扰物,可能掩盖真正的测试偏差.
- 精心选择EQA材料对于准确评估实验室测定性能和可变性至关重要.
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