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暴露于pyrifos干扰了在猪卵细胞成熟期间的卵细胞复合体中的细胞间通信

Yajie Chu¹, Qiao Li¹, Yijing He¹

¹MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, China.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

|April 2, 2024

概括

pyrifos农药暴露破坏了卵细胞的细胞间通信,导致成熟失败. 这种杀虫剂损害了累积膨胀,诱导氧化应激和亡,并影响差距连接和跨区域投射.

科学领域:

生殖生物学生殖生物学
毒理学毒理学毒理学
细胞生物学细胞生物学

背景情况:

有机类农药,如酸 (CPF) 是广泛使用的,但具有生殖风险.
积卵细胞复合体 (COCs) 内的细胞间通信对于卵细胞成熟至关重要.
CPF对COC细胞间通信的具体影响仍未得到充分研究.

研究的目的:

为了研究CPF对猪在体外成熟过程中对猪的COC的影响.
阐明CPF干扰累积细胞 (CCs) 和卵细胞之间的通信的机制.
评估CPF对COC扩张,半分裂,亡,氧化应激以及隙结和跨区域投射等结构部件的影响.

主要方法:

猪的COC在体外成熟过程中暴露于CPF.
评估的是第一个极体挤出率和介质进展率.
评估的是累积扩张,基因表达 (累积扩张,抗氧化酶),亡标志物,活性氧物种 (ROS) 水平,线粒体膜潜力 (MMP),间隙结 (GJ) 功能 (素,连xin43) 和跨区域投射 (TZPs).

主要成果:

暴露于CPF显著降低了第一个极体挤出率,并阻断了半分裂.
累积扩张被抑制,相关基因的mRNA水平下调.
在CCs中,CPF增加了早期亡,改变了BAX/BCL2和分裂的caspase3表达,并诱导了氧化应激 (增加ROS,减少MMP).
间隙结功能和连接素43的表达发生了变化,而跨区域的投射减少了.
暴露于CPF导致卵细胞成熟受损.

结论:

хлорпирифос抑制累积膨胀,并影响卵细胞成熟.
在累积细胞中,CPF会诱导氧化应激和亡.
杀虫剂破坏了关键的细胞间通信通路,包括间隙结和跨区域投射,最终导致卵细胞成熟失败.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.基基酸盐是一种基基酸盐.累积细胞-卵细胞复合体.差距差距的交叉点差距的交叉点跨区域预测跨区域预测

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