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Updated: Jun 29, 2025

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马拉巴 (Epinephelus malabaricus) 的功能性特征干扰素调节因子9参与抗病毒反应

Thirunavukkarasu Periyasamy¹, Lu Ming-Wei², Sharmila Velusamy³

¹Laboratory of Molecular Virology and Immunology, Department of Aquaculture, The College of Life Science, National Taiwan Ocean University, Keelung 202, Taiwan; Department of Biotechnology, Nehru Arts and Science College, Coimbatore 641105, Tamil Nadu, India.

International journal of biological macromolecules

|April 2, 2024

概括

马拉巴群IRF9 (干扰素调节因子9) 对于抗病毒防御至关重要. 这项研究揭示了它在干扰素信号传递和基因表达中的作用,这对群的免疫力至关重要.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
水产养殖是水产养殖的一种方式.

背景情况:

干扰素调节因子9 (IRF9) 是JAK-STAT通路的关键,形成ISGF3驱动干扰素刺激基因 (ISG) 转录.
马拉巴群 (Epinephelus malabaricus) 中通过干扰素信号调节IRF9的具体机制尚不清楚.

研究的目的:

为了研究马拉巴群IRF9 (MgIRF9) 的组织特异性mRNA分布.
为了阐明MgIRF9在E. malabaricus中的抗病毒功能和调节机制.
探索MgIRF9在鱼中干扰素信号通路中的相互作用.

主要方法:

分析了MgIRF9mRNA在各种群群组织中的分布.
使用神经亡病毒和多 (I:C) 来评估MgIRF9表达的诱导研究.
在体外凝移动性转移试验中,检查MgIRF9与zfIFN促进体的结合.
重组干扰素治疗和MgIRF9在GK细胞中的过度表达实验.
同免疫沉降证实MgIRF9和STAT2.2之间的体内相互作用.

主要成果:

MgIRF9mRNA存在于所有组织中,在肌肉,和大脑中含量最高.
在感染神经亡病毒和多 (I:C) 刺激后,MgIRF9的表达显著增加.
MgIRF9与zfIFN促进体结合,并增强干扰素和ISG (ADAR1,ADAR1-Like,ADAR2) 的表达.
在体内,MgIRF9与STAT2相互作用,这表明它在ISGF3复合体形成中的作用.

结论:

马拉巴群IRF9在干扰素信号通路中发挥着重要作用.
MgIRF9参与诱导ISG基因表达,以应对病毒感染和干扰素刺激.
这项研究提供了对鱼种类天生的免疫反应机制的见解.

关键词:

亚达尔家庭的家庭.上腺虫 (Epinephelus malabaricus) 是一种有毒的动物.在 IRF9 中,IRF9 是 IRF9 的第一个类型.干扰素干扰素干扰素马拉巴尔的小鱼是马拉巴尔的小鱼.神经亡病毒的神经亡病毒

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