通过notoginsenoside R1抑制JAK2/STAT3通路减少了非小细胞肺癌中的上皮层-介质细胞过渡
Min Wan1, Hong Yu1, Haoqing Zhai2
1Department of Medical Laboratory, Wuhan Hospital of Traditional Chinese Medicine, Wuhan, 430014, China.
Molecular biotechnology
|April 2, 2024
概括
诺托金化物R1 (NGR1) 有效地抑制非小细胞肺癌 (NSCLC) 的生长和转移. NGR1通过禁用JAK2/STAT3信号通路来抑制上皮层-介质细胞转换 (EMT) 过程,显示NSCLC的治疗潜力.
科学领域:
- 在瘤学瘤学.
- 药理学 药理学是指药理学的学科.
- 分子生物学分子生物学
背景情况:
- 非小细胞肺癌 (NSCLC) 仍然是全球癌症死亡的主要原因.
- 迫切需要针对NSCLC进展的新型治疗剂.
- 一种沙宁的Notoginsenoside R1 (NGR1) 已经显示出潜在的抗瘤活性.
研究的目的:
- 研究NGR1在非小细胞肺癌 (NSCLC) 中的抗癌作用和潜在机制.
- 评估NGR1对NSCLC细胞活力,增殖,迁移和侵入性的影响.
- 阐明NGR1对表皮细胞-介质细胞过渡 (EMT) 和NSCLC中JAK2/STAT3通路的影响.
主要方法:
- 使用ex vivo测试来评估NSCLC细胞行为 (活力,增殖,迁移,侵入性).
- 建立了NSCLC异种移植小鼠模型用于体内验证.
- 免疫洗和免疫组织化学分析了EMT标记物和JAK2/STAT3通路蛋白.
主要成果:
- NGR1显著抑制NSCLC细胞生长,无论是体外还是体内.
- NGR1降低了NSCLC细胞的迁移和入侵潜力.
- NGR1通过增加E-cadherin和减少N-cadherin,vimentin和牛表达来抑制EMT,同时也使JAK2/STAT3通路失活.
结论:
- NGR1通过抑制上皮细胞-介质细胞转换 (EMT) 过程,表现出强大的抗NSCLC作用.
- 该机制涉及到JAK2/STAT3信号通路的失活.
- NGR1代表了非小细胞肺癌治疗的有希望的治疗候选者.
相关概念视频
01:20The JAK-STAT Signaling Pathway
8.9K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
8.9K
03:03mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
02:23Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
01:19Interactions Between Signaling Pathways
6.3K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.3K
01:20The Retinoblastoma Gene
4.1K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.1K
01:16TGF - β Signaling Pathway
7.3K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.3K