探索ex vivo生物膜动态:低西林度对人类口腔微生物群的影响
N K Brar1, A Dhariwal1, H A Åmdal1
1Institute of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway.
NPJ biofilms and microbiomes
|April 2, 2024
概括
在口服生物膜中低剂量安比西林暴露可以增加细菌的存活率并改变微生物群落. 这项研究揭示了影响抗生素耐药性基因 (ARG) 在口腔微生物群中的丰富性和多样性的复杂相互作用.
科学领域:
- 微生物学 微生物学
- 口腔微生物组研究
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 长时间暴露于低度抗生素可以驱动细菌生物膜的形成,毒性和抗生素耐药性.
- 口腔微生物组含有大量抗生素耐药性基因 (ARG),这是由于共生动物和病原体的共存.
研究的目的:
- 为了研究安皮西林对人类口服生物膜活性的影响.
- 使用ex vivo模型分析对口腔微生物群和抗体的生态影响.
主要方法:
- 使用人类口腔生物膜的ex vivo模型.
- 采用枪元基因组学来评估微生物和抗体样本.
- 量化生物膜活力,微生物分类学概况以及ARG的多样性和丰富性.
主要成果:
- 低度的安皮西林显著改变了微生物的分类学特征,并提高了生物膜的活力1-2 log.
- 没有观察到对ARG多样性的重大影响,但ARG丰度降低了高达84%.
- 发现微生物多样性减少和ARG丰度减少之间存在正相关性. 链球菌,包括Streptococcus mitis,显示了ARG的增加.
结论:
- 活体口服生物膜模型有效地证明了安皮西林对微生物组和抗体组动态的影响.
- 低剂量的安比西林可以促进生物膜活力和改变微生物组成,对ARG产生复杂的影响.
- 这些发现强调了口腔链球菌在ARG传播中的作用,并强调了进一步研究抗菌素耐药性驱动因素的必要性.
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