在61种水生细菌分离物中对抗微生物耐药性的基因组表征
Chrissy D Eckstrand1,2, Brandi K Torrevillas1, Rebecca M Wolking1
1Washington Animal Disease Diagnostic Laboratory, College of Veterinary Medicine, Washington State University, Pullman, WA, USA.
概括
鱼类病原体中的抗菌素耐药性 (AMR) 是一个越来越令人担忧的问题. 全基因组测序为研究水生动物抗菌耐药性的新方法提供了机会,克服了传统测试方法的挑战.
科学领域:
- 水生动物的健康状况.
- 微生物学 微生物学
- 基因组学就是基因组学.
背景情况:
- 水生病原体中的抗菌素耐药性 (AMR) 尚未得到研究.
- 对水产养殖和野生鱼种群来说,抗菌剂的使用至关重要.
- 传统的抗微生物敏感性测试对水生细菌具有挑战性.
研究的目的:
- 用全基因组测序来研究鱼类病原体中的AMR.
- 描述AMR基因,预测耐药性表型和毒性因子.
- 评估初步细菌鉴定的准确性.
主要方法:
- 来自鱼类的61种细菌分离物的全基因组测序.
- 对AMR基因和毒性因子的组装基因组草案的分析.
- 与由矩阵辅助激光脱/飞行时间质谱学所做的初步鉴定进行比较.
主要成果:
- 组装的基因组草稿,AMR基因,预测的耐药性表型和病毒性因素被确定.
- 这项研究提供了来自鱼类的61种细菌分离物的基因组数据.
- 讨论了基因组数据和初步鉴定之间的一致性.
结论:
- 全基因组测序是一种可行的方法,用于研究水生动物病原体中的AMR.
- 基因组数据可以帮助监测AMR和了解细菌毒性.
- 这项研究有助于FDA CVM Vet-LIRN监测AMR的努力.
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