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Updated: Jun 29, 2025

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Live Imaging of Mouse Secondary Palate Fusion
Published on: July 27, 2017
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来自人类宫腔介质细胞的外体细胞通过促进口腔上皮细胞迁移,在宫腔融合过程中调解细胞间通信
Zhuo Huang1, Yusheng Zhi1, Haiyan Cao1
1State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei, People's Republic of China.
International journal of nanomedicine
|April 3, 2024
概括
胚胎 palatal 细胞的外体细胞在发育过程中影响口腔上皮细胞的行为. 这些来自细胞的囊泡调节了增殖和迁移,为 palatal 融合机制提供了洞察力.
科学领域:
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
- 细胞外囊泡 细胞外囊泡
背景情况:
- 外体在细胞间通信中起到关键的调解作用.
- 外体在 palatogenesis 中的作用,特别是表皮-介质细胞相互作用中,仍然在很大程度上未被探索.
研究的目的:
- 研究从人类胚胎 palatal mesenchymal (HEPM) 细胞中衍生出来的外体的功能和机制,在 palatogenesis 过程中调节上皮层-mesenchymal 通信.
- 探索HEPM衍生的外体对口腔上皮细胞行为的影响.
主要方法:
- 免疫光染色检测出 palatal 细胞中的外体体标记物 (CD63,CD81).
- 人类胚胎 palatal mesenchymal (HEPM) 细胞衍生外体 (HEPM-EXO) 被分离并使用NTA,TEM和Western blot进行了表征.
- HEPM-EXO与人类永生口腔上皮细胞 (HIOEC) 共同培养,以评估对增殖,迁移,亡和上皮-介质细胞过渡 (EMT) 的影响.
- 蛋白质组分析确定了HEPM-EXO中的蛋白质.
主要成果:
- 外基因组标记物CD63和CD81在发育过程中在 palatal 细胞中得到广泛的表达 (E12.5-E14.5).
- HEPM-EXO被HIOEC内部化,表明它们有能力在组织边界之间调解细胞-细胞通信.
- HEPM-EXO显著抑制了HIOEC的扩散,并刺激了迁移,而不会影响亡或EMT.
- 蛋白质组分析确定THBS1是HIOEC行为的潜在调节者,并揭示了HEPM-EXO中与伤口愈合相关的蛋白质.
结论:
- 从 palatal 介质细胞中衍生出来的 exosomes 在 palatogenesis 中发挥作用,通过调解细胞-细胞通信.
- HEPM-EXO调节口腔上皮细胞的增殖和迁移,这表明了协调组织发育的机制.
- 这些发现暗示了HEPM-EXO在组织修复和再生中的潜在治疗应用.
关键词:
皮质-介质细胞相互作用细胞外囊泡中的细胞外囊泡.palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis palatogenesis Palatogenesis是什么意思呢? palatogenesis是什么意思呢? palatogenesis是什么意思蛋白质组学 蛋白质组学伤口愈合 伤口愈合相关概念视频
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