相关实验视频

Updated: Jun 29, 2025

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

Published on: January 7, 2020

在巨细胞中通过通过TLR4/NF-κB通路通过脂多糖酸激活血管原蛋白表达在结肠炎中

Zhengrong Yao^1,2, Rongpan Bai^1,2, Wei Liu³

¹Institute of Environmental Medicine and Department of General Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China.

Acta biochimica et biophysica Sinica

|April 3, 2024

概括

科学家们在炎症性肠病 (IBD) 中发现了一条新的调节途径. 脂聚糖 (LPS) 激活托尔类受体4 (TLR4) 和NF-κB,以增加巨细胞中的血管新生素 (ANG),控制炎症.

科学领域:

免疫学免疫学免疫学
胃肠病学胃肠病学
分子生物学分子生物学

背景情况:

炎症性肠病 (IBD) 给我们的健康带来了重大挑战.
巨细胞通过分泌细胞因子和调节组织修复,在IBD中发挥着至关重要的作用.
在IBD中,巨细胞衍生的血管新生素 (ANG) 的上游调节和特定功能在很大程度上是未知的.

研究的目的:

阐明控制巨细胞中血管新生素 (ANG) 表达的调节途径.
在炎症性肠病 (IBD) 的背景下研究 ANG 的作用.

主要方法:

在大肠炎和脂多糖化物 (LPS) 刺激后,研究了巨细胞中的ANG表达.
利用分子生物学技术来识别参与ANG调节的信号通路.
评估了ANG删除对巨细胞功能 (如细胞和化学反应) 的影响.

主要成果:

在大肠炎和LPS治疗期间,巨细胞的ANG表达显著上调.
脂聚糖-托尔类受体4 (LPS-TLR4) 途径激活NF-κB,从而增强ANG转录.
巨细胞特异性缺失ANG并没有损害巨细胞的基本功能.

结论:

在炎症性疾病中发现了一种新的"LPS-TLR4-NF-κB-ANG"调节轴.
血管新生素 (ANG) 以一种近的方式起作用,调节炎症.
ANG被证实是炎症反应网络中的关键调解者.

关键词:

在 NF-κBB 中.在TLR4中,血管新生素的产生脂多糖类糖化物 (Lipopolysaccharide) 是一种脂多糖的化合物.巨细胞是一个巨细胞.

更多相关视频

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells

Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells

Published on: September 18, 2016

相关概念视频

NF-κB-dependent Signaling Pathway

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...