相关实验视频
Updated: Jun 29, 2025

08:07
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
Published on: April 12, 2019
7.2K
亚洲酸通过AKT/Sp1/MMP1轴抑制骨肉瘤细胞迁移和侵入
Yat-Yin Law1,2,3, Hsiang-Lin Lee1,4, Chu-Liang Lin3
1School of Medicine, Chung Shan Medical University, Taichung, Taiwan.
Environmental toxicology
|April 3, 2024
概括
亚洲酸 (AA) 通过抑制癌细胞迁移和入侵,显示出对骨髓瘤的潜力. 它针对p-AKT/Sp1/MMP1通路,为治疗这种骨癌提供了新的途径.
科学领域:
- 在瘤学瘤学.
- 药理学 药理学是指药理学的学科.
- 分子生物学分子生物学
背景情况:
- 骨髓瘤是儿童和青少年患有有限治疗选择的流行骨癌.
- 来自Centella asiatica的亚洲酸 (AA) 在各种癌细胞类型中表现出已知的抗瘤特性.
- 由于缺乏有效的治疗方法,对骨髓瘤的新疗法进行研究至关重要.
研究的目的:
- 评估亚洲酸 (AA) 对人类骨髓瘤细胞的抗瘤作用.
- 阐明AA在骨髓瘤中的作用背后的分子机制.
- 探索AA作为治疗骨髓瘤的治疗剂的潜力.
主要方法:
- 进行了细胞活力,增殖和细胞周期测试.
- 进行了细胞迁移和入侵试验.
- 使用了基因表达分析 (MMP1,Sp1) 和西式涂抹 (p-AKT).
- 对TNMplot数据库进行了生物信息分析.
主要成果:
- 亚洲酸 (AA) 没有影响骨髓瘤细胞活力,增殖或细胞周期.
- 通过降低MMP1表达的调节,AA显著抑制骨髓瘤细胞迁移和入侵.
- AA降低了Sp1表达和AKT酸化,这是p-AKT/Sp1/MMP1通路的关键组成部分.
- 过度表达MMP1逆转了AA的抗迁移和抗侵入作用,而过度表达Sp1则取消了AA的疗效.
结论:
- 亚洲酸 (AA) 对人类骨髓瘤细胞表现出抗侵入性和抗迁移性作用.
- AA的抗癌活性通过p-AKT/Sp1/MMP1信号通路进行介导.
- 酸盐作为骨髓瘤治疗的潜在治疗候选人具有前景.
相关概念视频
mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
Cancer Cell Migration through Invadopodia
2.3K
Invadosome is a broad category of cell surface structures with proteolytic activity that degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
2.3K
The JAK-STAT Signaling Pathway
8.9K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
8.9K
PI3K/mTOR/AKT Signaling Pathway
3.5K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.5K

