在pH值变化引起的变化后的变质化过程中,对大豆11S环球蛋白的结构和功能性质的表征
Yang Yang1, Can Zhang1, Chun-Min Ma1
1College of Food Engineering, Harbin University of Commerce, Harbin, China.
Journal of the science of food and agriculture
|April 3, 2024
概括
研究了大豆11S环球蛋白在不同pH值的变性和重变性. 酸性和性处理改变了蛋白质的结构和功能,有可能增强大豆蛋白产品的开发.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 生物化学 生物化学
背景情况:
- 11S环球蛋白是一种关键的大豆蛋白,具有多样化的功能性质.
- 了解其变质和重新变质对于优化大豆蛋白应用至关重要.
研究的目的:
- 探索在pH3.0和10.0的脱化过程中11S环球蛋白的结构和功能变化,然后在pH7.0的重新化.
- 在不同的pH条件下阐明11S环球蛋白的稳定机制.
主要方法:
- 在酸性 (pH 3.0) 和性 (pH 10.0) 条件下11S环球蛋白的变性.
- 通过逐步恢复到中性pH (7.0) 的方法重新化.
- 分析结构变化 (三级,二级结构) 和功能性质 (溶解度,发泡能力,乳化活性指数).
主要成果:
- pH诱导的变和随后的重导致11S环球蛋白的三级结构的部分重新折叠.
- 二次结构 (α-螺旋,β-叶) 和颗粒大小显示在再生过程中与原生蛋白逐渐对齐.
- 与原始状态相比,化化11S环球蛋白表现出增加的发泡能力和乳化活性指数,在再生后功能性质得到改善.
- 酸化11S环球蛋白在重化过程中显示出β-片含量和功能性质的明显变化.
结论:
- 该研究提供了在不同的pH条件下11S环球蛋白的结构和功能修饰的见解.
- 这些发现为开发功能增强的大豆蛋白产品提供了理论基础.
- 这项研究扩大了大豆蛋白成分的潜在应用.
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