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电泳沉积介射测量散射质量摄影学

Matthew D Kowal¹, Teresa M Seifried¹, Carraugh C Brouwer¹

¹Department of Chemistry, University of British Columbia, Vancouver, British Columbia V6T 1Z1, Canada.

|April 3, 2024

概括

电泳沉积干扰散射显微镜 (EPD-iSCAT) 使用电场快速沉积纳米粒子到覆盖上. 这种方法克服了缓慢的扩散极限,使得精确的,实时监测纳米颗粒度和溶液中的大小.

科学领域:

纳米技术纳米技术
生物物理学的生物物理.
分析化学分析化学

背景情况:

介面计散射显微镜 (iSCAT) 是一种无标签的技术,用于分析单个纳米粒子和宏分子.
iSCAT通过分析颗粒物如何降落和吸附到覆盖上来测量颗粒体积和溶液度.
iSCAT的一个主要限制是颗粒对测量表面的缓慢扩散率,限制了测量频率.

研究的目的:

开发一种方法,以克服干涉度散射显微镜中的扩散有限采样率.
引入电泳沉积干扰散射显微镜 (EPD-iSCAT) 进行增强的纳米粒子分析.
为了证明用户对粒子沉积率的控制,用于改进iSCAT测量.

主要方法:

EPD-iSCAT使用一种导电性氧化物 (ITO) 涂层的盖片.
将电压应用于 ITO 覆盖滑动器会产生电场,驱动带电纳米粒子的电泳沉积.
沉积速度是通过调节应用电压来控制的.

主要成果:

通过EPD-iSCAT,可以控制纳米粒子沉积,克服缓慢扩散的限制.
对于50nm聚乙烯纳米颗粒,在0.1nM度和+1V潜力下,达到1.7s−1 μm−2 nM−1的沉积速率常数.
在移除应用电压后,沉积停止,证明了精确的控制.

结论:

对于iSCAT测量,EPD-iSCAT显著提高了纳米粒子沉积的速度和控制.
这种技术允许使用单分子质量摄影仪长期监测溶液中的缓慢运动过程.
EPD-iSCAT提供了依赖度的沉积率,类似于传统方法,但增加了电泳控制.

关键词:

电泳性沉积是一种电泳性沉积.干扰计散射显微镜的干扰计散射显微镜.机器学习是机器学习.质量测光仪的质量测光仪.定量成像技术定量成像技术单个纳米粒子检测检测

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