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相关概念视频

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

765
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
765
Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

809
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
809
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

730
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
730
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

458
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
458
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

310
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI spectrometry is widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.
The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix material. The...
310
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

230
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
230

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相关实验视频

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Rapid Determination of Antibody-Antigen Affinity by Mass Photometry
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电泳沉积 介射测量 散射质量 摄影学

Matthew D Kowal1, Teresa M Seifried1, Carraugh C Brouwer1

  • 1Department of Chemistry, University of British Columbia, Vancouver, British Columbia V6T 1Z1, Canada.

ACS nano
|April 3, 2024
PubMed
概括

电泳沉积干扰散射显微镜 (EPD-iSCAT) 使用电场快速沉积纳米粒子到覆盖上. 这种方法克服了缓慢的扩散极限,使得精确的,实时监测纳米颗粒度和溶液中的大小.

关键词:
电泳性沉积是一种电泳性沉积.干扰计散射显微镜的干扰计散射显微镜.机器学习是机器学习.质量测光仪的质量测光仪.定量成像技术 定量成像技术单个纳米粒子检测检测

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科学领域:

  • 纳米技术纳米技术
  • 生物物理学的生物物理.
  • 分析化学 分析化学

背景情况:

  • 介面计散射显微镜 (iSCAT) 是一种无标签的技术,用于分析单个纳米粒子和宏分子.
  • iSCAT通过分析颗粒物如何降落和吸附到覆盖上来测量颗粒体积和溶液度.
  • iSCAT的一个主要限制是颗粒对测量表面的缓慢扩散率,限制了测量频率.

研究的目的:

  • 开发一种方法,以克服干涉度散射显微镜中的扩散有限采样率.
  • 引入电泳沉积干扰散射显微镜 (EPD-iSCAT) 进行增强的纳米粒子分析.
  • 为了证明用户对粒子沉积率的控制,用于改进iSCAT测量.

主要方法:

  • EPD-iSCAT使用一种导电性氧化物 (ITO) 涂层的盖片.
  • 将电压应用于 ITO 覆盖滑动器会产生电场,驱动带电纳米粒子的电泳沉积.
  • 沉积速度是通过调节应用电压来控制的.

主要成果:

  • 通过EPD-iSCAT,可以控制纳米粒子沉积,克服缓慢扩散的限制.
  • 对于50nm聚乙烯纳米颗粒,在0.1nM度和+1V潜力下,达到1.7s−1 μm−2 nM−1的沉积速率常数.
  • 在移除应用电压后,沉积停止,证明了精确的控制.

结论:

  • 对于iSCAT测量,EPD-iSCAT显著提高了纳米粒子沉积的速度和控制.
  • 这种技术允许使用单分子质量摄影仪长期监测溶液中的缓慢运动过程.
  • EPD-iSCAT提供了依赖度的沉积率,类似于传统方法,但增加了电泳控制.