酵母细胞反应和周期性透应激期间的存活率由葡萄糖的可用性控制
Fabien Duveau1,2, Céline Cordier3, Lionel Chiron3
1Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS & Université Paris Diderot, 10 rue Alice Domon et Léonie Duquet, Paris, France.
eLife
|April 3, 2024
概括
细胞对动态环境变化的反应取决于压力时间. 酵母细胞在透应激和葡萄糖剥夺相处时繁荣发展,突出了新陈代谢在应激适应中的作用.
科学领域:
- 细胞生物学 细胞生物学
- 环境压力反应环境压力反应
- 酵母遗传学 酵母遗传学
背景情况:
- 细胞环境是动态的,具有波动的参数,如透应激和营养的可用性.
- 了解细胞如何对多个同时变化的因素做出反应,对于细胞生物学来说至关重要.
研究的目的:
- 量化化应激和葡萄糖剥夺的双重周期性波动对酵母细胞行为的影响.
- 研究应激分相 (在相相相对替代) 对细胞增殖,存活和信号传递的作用.
主要方法:
- 利用微流体来精确控制环境条件.
- 采用时间缩短显微镜,随着时间的推移监测酵母细胞反应.
- 量化细胞增殖,生存率和转录反应.
主要成果:
- 当透应激和葡萄糖剥夺发生在相位时,细胞增殖和存活率显著提高,而不是相反相位.
- 阶段内压力导致酵母细胞的转录反应下降.
- 具有受损透应激反应的突变体比野生类型细胞更好地适应交替应激.
结论:
- 葡萄糖的可用性动态调节酵母对透应激的反应,强调了代谢波动的重要性.
- 在动态相互作用的压力条件下,对持续压力的遗传适应可能是不利的.
- 环境压力因素的分阶段是细胞命运和反应的关键决定因素.
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