开发和验证一种GC-MS/MS方法,用于在生物样本中测定酸
Hanning Yu1,2, Linying Wu3, Dongliang Xuan3
1Centers for Water and Health, Key Laboratory of Public Health Safety, Ministry of Education, Fudan University, Shanghai, 200032, China.
Analytical and bioanalytical chemistry
|April 3, 2024
概括
酸 (IAA) 是一种有毒的消毒副产品,在摄入后在各种老鼠器官中检测到. 这项研究提供了关于IAA的第一个数据.
科学领域:
- 环境化学环境化学
- 毒理学 毒理学 毒理学
- 分析化学 分析化学
背景情况:
- 酸 (IAA) 是一种消毒副产品,具有显著的细胞毒性,基因毒性和内分泌干扰潜力.
- 关于哺乳动物摄入后IAA的体内分布和分泌的数据有限.
- 由于其潜在的健康风险,了解IAA的毒理特征至关重要.
研究的目的:
- 开发和验证一种在生物样本中检测酸 (IAA) 的方法.
- 在口服暴露后调查哺乳动物体内IAA的分布和分泌.
- 为风险评估提供IAA毒动力学方面的关键数据.
主要方法:
- 修改的QuEChERS样品准备技术.
- 气体染色学-并联三重四极质谱学 (GC-MS/MS) 检测.
- 生物矩阵的分析,包括血,尿液,便,肝脏,脏和组织.
主要成果:
- 一种可靠和验证的方法,具有令人满意的恢复率 (62.6-108.0%) 和低RSD (<12.3%).
- 在所有生物矩阵 (0.007-0.032 ng/g) 中,IAA的低检测极限.
- 在大鼠血,甲状腺,肝脏,脏,脏和胃肠道中检测到IAA6小时后.
结论:
- 开发的GC-MS/MS方法对于生物样本的IAA分析是可靠和可重复的.
- 这项研究提供了第一个关于口服暴露后哺乳动物IAA分布和分泌的体内数据.
- 这些发现突显了IAA的系统分布,为未来的毒理学和风险评估研究提供了信息.
相关概念视频
Gas Chromatography–Mass Spectrometry (GC–MS)
Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall. The coating...
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall. The coating...
Peptide Identification Using Tandem Mass Spectrometry
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...


