代谢工程Corynebacterium glutamicum用于D-chiro-inositol生产的代谢工程
Guohui Ji1,2, Xia Jin1,2, Feng Shi3,4
1Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.
World journal of microbiology & biotechnology
|April 3, 2024
概括
研究人员设计了一种Corynebacterium glutamicum菌株,用于增强D-chiro-inositol (DCI) 生产. 优化的菌株达到6.96g/L的DCI标位纪录,显示出治疗II型糖尿病和PCOS的前景.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 微生物发酵 微生物发酵
背景情况:
- D-chiro-inositol (DCI) 是一种有价值的化合物,具有治疗II型糖尿病和多囊性卵巢综合征的治疗潜力.
- 高效的微生物合成DCI对于其制药应用至关重要.
研究的目的:
- 为高产量DCI生产设计一种Corynebacterium glutamicum菌株.
- 为了优化发酵条件以最大限度地提高DCI标位.
主要方法:
- 通过敲除C. glutamicum中的内醇代谢基因,产生了一个底盘菌株 (DCI-1).
- 在底盘菌株中表达了各种异酶和异醇脱酶 (IolG).
- 优化发酵参数,包括pH值,温度和基质度.
主要成果:
- 工程菌株DCI-7,共表达IolI2和IolG,达到3.21g/L的DCI标位,使用20g/L的myo-inositol (MI).
- 进一步优化导致在特定条件下 (pH 8.0,37°C,40 g/L MI) 的最大DCI标位为6.96 g/L.
- 这代表了使用全细胞生物转换报告的最高DCI标位.
结论:
- 经过工程设计的C. glutamicum菌株DCI-7证明了DCI生产的高效和可扩展的方法.
- 取得的DCI标位显著提高了这种治疗化合物的工业规模合成潜力.
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