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相关实验视频

Updated: Jun 29, 2025

Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments

Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments

Published on: July 10, 2013

来自败血症小鼠的压力粒细胞识别的协议.

Yu Hao¹, Can Zhang¹, Fangyuan Li¹

¹Biomedical Innovation Center, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China; Beijing Key Laboratory for Therapeutic Cancer Vaccines, Beijing 100038, China.

|April 3, 2024

概括

败血症会激活称为粒细胞的免疫细胞,增强它们对随后感染的反应. 这项研究详细介绍了一种小鼠模型来研究这种现象及其对炎症和生存的影响.

关键词:

流动细胞计量 (Flow Cytometry) 是一种流动细胞计.免疫学免疫学免疫学模型生物模型生物

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Last Updated: Jun 29, 2025

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科学领域:

免疫学免疫学免疫学
微生物学微生物学
传染性疾病传染性疾病

背景情况:

败血症可以改变免疫细胞的功能,可能会影响对后续感染的反应.
颗粒细胞在对细菌病原体的天生的免疫反应中发挥着关键作用.

研究的目的:

提出一个可复制的小鼠模型来研究败血症,其次是二次感染.
调查败血症后颗粒细胞的功能变化及其对宿主防御的影响.

主要方法:

建立一个小鼠模型,使用结和穿孔 (CLP) 来诱导败血症.
在康复阶段,用脂多糖 (LPS) 或Pseudomonas aeruginosa重新挑战小鼠.
颗粒细胞表型的表征和其功能能力的评估.

主要成果:

开发的模型允许研究细粒细胞中败血症诱导的免疫训练.
败血症条件下的粒细胞表现出改变的功能表型.
颗粒细胞的功能状态影响了面临二次感染的小鼠的死亡率.

结论:

该协议为研究败血症,粒细胞功能和二次感染之间的复杂相互作用提供了有价值的工具.
了解细粒细胞中败血症诱导的免疫调节对于开发有针对性的疗法以改善患者的治疗结果至关重要.