相互透的凝/酸盐混合水凝:最简单的方法来制备一个可自闭合的脚手架
Hideki Mori1, Yaya Taketsuna1, Kae Shimogama1
1Department of Biological Chemistry, Graduate School of Science, Osaka Metropolitan University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Japan.
Journal of bioscience and bioengineering
|April 3, 2024
概括
可自的凝/甲基酸盐水凝为细胞培养提供了一种简单的方法. 这些凝支持人类骨髓瘤细胞的附着和增殖,使它们成为有希望的生物相容培养基.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 细胞生物学 细胞生物学
背景情况:
- 水凝灭菌方法对细胞培养应用的准备容易产生影响.
- 在先进的细胞培养技术中,开发强大且可灭菌的水凝至关重要.
研究的目的:
- 开发一种简单的方法来制备可自闭合的凝/酸水凝.
- 为了表征这些水凝在自封化后的物理和机械性能.
- 为了评估细胞培养水凝的细胞相容性.
主要方法:
- 通过成型凝/甲基酸盐混合物,然后进行化化的化.
- 测量了水凝的特性,包括体积,扬模量,储存模量和损失模量.
- 人类骨髓瘤 (HOS) 细胞在水凝上培养,以评估附着和增殖.
主要成果:
- 这种成型方法提供了一条简单的途径,可自闭合的凝/酸水凝.
- 自动缩导致水凝收缩,允许基于体积减小比率的形状设计.
- 高度的凝/甲基酸盐水凝在自封后表现出很低的变形和最小的凝化.
- 在水凝上观察到HOS细胞的显著附着和增殖.
结论:
- 使用简单的成型技术,可以轻松地制造出 желатин/酸的水凝,使其具有所需的形状.
- 这些水凝是可自的,保持结构完整性并支持细胞生长.
- 由于其稳定性和细胞相容性,高度的凝/甲基酸盐水凝适合作为自培养基.
相关概念视频
Cohesion
44.9K
Cohesion is the attraction between molecules of the same type, such as water molecules. Water molecules have an overall neutral charge but are polar molecule. An oxygen atom in one water molecule has a partial negative charge that can bind to a hydrogen atom with a partial positive charge in a second water molecule, forming a hydrogen bond. Each water molecule can form up to four hydrogen bonds with other water molecules. Hydrogen bonds are responsible for water's cohesive nature.
On a...
On a...
44.9K
Dehydration Synthesis
133.4K
Overview
Dehydration synthesis (also called a condensation reaction) is the chemical process in which two molecules covalently link together to form a new molecule, along with the release of a water molecule. Many physiologically important compounds form by dehydration synthesis reactions, such as complex carbohydrates, proteins, DNA, and RNA.
Synthesis of carbohydrates
Sugar molecules are covalently linked together by dehydration synthesis. During the reaction, the hydroxyl (-OH) group from...
Dehydration synthesis (also called a condensation reaction) is the chemical process in which two molecules covalently link together to form a new molecule, along with the release of a water molecule. Many physiologically important compounds form by dehydration synthesis reactions, such as complex carbohydrates, proteins, DNA, and RNA.
Synthesis of carbohydrates
Sugar molecules are covalently linked together by dehydration synthesis. During the reaction, the hydroxyl (-OH) group from...
133.4K


