基于微平衡的石英晶体口味传感器与磁性预度系统集成,用于检测食品样本中的Listeria monocytogenes
Fatma Beyazit1, Mehmet Yakup Arica2, Ilkay Acikgoz-Erkaya3
1Department of Obstetrics and Gynecology, Faculty of Medicine, Çanakkale Onsekiz Mart University, Çanakkale, Turkey.
Mikrochimica acta
|April 3, 2024
概括
一种具有磁性预富的新型石英晶体微平衡 (QCM) 适应传感器可以快速准确地检测出Listeria monocytogenes. 该系统为食品安全应用提供了高的特异性和可重复性.
科学领域:
- 生物感知和纳米技术
- 食品安全与微生物学
背景情况:
- 准确和快速检测Listeria monocytogenes对于公共卫生和食品安全至关重要.
- 传统的细菌检测方法可能是耗时和劳动密集的.
- 开发敏感和特定的生物传感器对于有效的病原体识别至关重要.
研究的目的:
- 设计和描述一种新的石英晶微平衡 (QCM) 适应传感器,用于快速和特定检测Listeria monocytogenes.
- 开发一个磁性预丰富系统,用于在apt传感器检测之前的样品消毒和度.
- 为了评估开发的QCM感应传感器系统的分析性能,特异性和可重复使用性.
主要方法:
- 制造磁性纳米粒子 (Fe3O4@PDA@DA-PEG) 用于预富.
- 在QCM晶体上固定特定的aptamer以捕获目标.
- 集成磁性预丰富与QCM适应传感器用于定量检测Listeria monocytogenes.
主要成果:
- 磁性预丰富系统实现了对Listeria monocytogenes的选择性隔离,效率高达91.8%.
- QCM适应传感器显示了广泛的分析范围 (1.0 × 10^2到1.0 × 10^7 CFU/mL),检测极限较低 (148 CFU/mL).
- 胃感应器对其他Listeria物种和常见细菌具有很高的特异性,可再生性和可重复使用性超过95%.
结论:
- 开发的QCM吸收传感器与磁性预丰富相结合,为Listeria monocytogenes检测提供了快速,准确和特定的方法.
- 这种生物传感平台显示出在食品安全监测和临床诊断中应用的巨大潜力.
- 该系统的可重复使用性和高性能指标强调了其实用性的实用性.
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