Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

1.3K
A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...
1.3K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

201
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
201
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.0K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.0K
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

3.7K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
3.7K
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

834
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
834
Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule01:10

Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule

1.3K
In the AX proton spin system, proton A can sense the two spin states of a coupled proton X, resulting in a doublet NMR signal with two peaks of equal (1:1) intensity. When proton A is coupled to two equivalent protons (AX2 spin system), the spin states of each X can be aligned with or against the external field, creating three possible scenarios. This results in a 1:2:1  triplet signal, where the central peak corresponds to the chemical shift of A and is twice as large or intense as the...
1.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Two-year follow-up of a novel fully 3D-printed off-the-shelf humeral total shoulder arthroplasty prosthesis.

JSES international·2026
Same author

Within-household transmission risk of pulmonary tuberculosis in the era of universal antiretroviral therapy.

medRxiv : the preprint server for health sciences·2026
Same author

Motivation and pleasure deficits reflect altered network embedding of normal brain deviations in schizophrenia.

Schizophrenia research·2026
Same author

Targeting the H/KRAS α4-β6-α5 Allosteric Lobe with Macrocyclic Peptides.

ACS medicinal chemistry letters·2026
Same author

Regional HNSCC metabolomics reveals widespread changes to one-carbon metabolism and S-adenosylmethionine metabolism across tumour core, tumour edge and adjacent non-tumour tissues.

British journal of cancer·2026
Same author

Asking for help: the development of a simulation-based mental health application to enhance depression literacy, mental health communication, and help-seeking among Black autistic youth.

Frontiers in psychiatry·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
查看所有相关文章

相关实验视频

Updated: Jun 29, 2025

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST

Published on: November 2, 2018

12.1K

使用NMR光谱分析分析复杂的RAS-效应器相互作用.

Regina Strakhova1, Matthew J Smith2,3

  • 1Institute for Research in Immunology and Cancer, Université de Montréal, Montreal, QC, Canada.

Methods in molecular biology (Clifton, N.J.)
|April 3, 2024
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种新的NMR方法,以量化多种蛋白质如何竞争与RAS GTPases结合. 这项技术增强了我们对复杂的细胞信号通路和RAS相关疾病的理解.

关键词:
竞争 竞争 竞争 竞争 竞争效应器的结合方式核磁共振光谱法 (NMR) 是一种光谱法.在 PLCε-1-1 中.英国皇家空军 (RAF) 的飞行员.在 RAS GTPases 中,在 RBD 域名中,使用 RBD 域.

更多相关视频

Author Spotlight: Unveiling the Structural and Dynamic Aspects of Glycan Molecular Recognition
07:40

Author Spotlight: Unveiling the Structural and Dynamic Aspects of Glycan Molecular Recognition

Published on: May 17, 2024

1.2K
Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

1.9K

相关实验视频

Last Updated: Jun 29, 2025

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST

Published on: November 2, 2018

12.1K
Author Spotlight: Unveiling the Structural and Dynamic Aspects of Glycan Molecular Recognition
07:40

Author Spotlight: Unveiling the Structural and Dynamic Aspects of Glycan Molecular Recognition

Published on: May 17, 2024

1.2K
Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

1.9K

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 细胞信号传递 细胞信号传递

背景情况:

  • RAS GTPases是调节细胞功能的关键分子开关.
  • RAS效应因子相互作用对于正常发育和瘤发生至关重要.
  • 多个效应器经常竞争激活RAS,这表明复杂的信号动态.

研究的目的:

  • 开发和验证一种基于NMR的新方法,用于量化与RAS GTPases结合的竞争性效应因子.
  • 为研究RAS信号提供一个更生理相关的体外系统.

主要方法:

  • 利用了核磁共振 (NMR) 光谱学.
  • 开发了使用同位素标记的野生型KRAS和未标记的RAS协会 (RA) 或RAS结合域 (RBD) 的竞争效应因子 (ARAF,PLCε1) 的竞争试验.
  • 通过在特征化学转移时测量峰值强度来量化结合.

主要成果:

  • 成功演示了一种方法,可以直接量化GTPase与竞争性效应剂的结合.
  • 该试验允许同时评估多种相互作用.
  • 适用于研究小分子竞争,GEF/GAP领域和调节性酶.

结论:

  • 开发的NMR方法提供了一种强大的方法来研究多重RAS效应器相互作用.
  • 这种技术以更加综合的方式推进了对RAS信号的生物物理和系统层面的理解.
  • 让体外测试更接近复杂的细胞环境,帮助研究RAS驱动的疾病.