Circ_0002669通过直接与MYCBP结合和海绵化miR-889-3p促进骨肉瘤瘤发生

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Ying Zhang^1,2, Yizhou Zhan³, Zhaoyong Liu⁴

¹Department of Radiotherapy, Cancer Hospital of Shantou University Medical College, No. 7 Raoping Road, 515041, Shantou, Guangdong, PR China. yzhangmimazi@stu.edu.cn.

|April 3, 2024

概括

循环RNAs (circRNAs) 促进骨髓瘤 (OS) 恶性. Circ_0002669保护MYCBP免受降解,并阻断miR-889-3p,推动OS细胞生长和入侵.

科学领域:

分子生物学分子生物学
在瘤学瘤学.
在RNA生物学,RNA生物学.

背景情况:

循环RNAs (circRNAs) 是癌症进展的关键调节者.
骨髓瘤 (OS) 是一种主要的骨癌,具有显著的死亡率.
特定的circRNAs,如circ_0002669,涉及到OS的发展.

研究的目的:

调查circ_0002669在骨髓瘤 (OS) 进展中的作用.
阐明circ_0002669影响OS恶性瘤的分子机制.
确定OS治疗的潜在治疗点.

主要方法:

定量实时PCR用于评估OS组织中的circ_0002669表达.
生物素拉下测试和质谱测试以确定circ_0002669-binding蛋白质.
路西法酶记者测定和RNA免疫沉以确认circ_0002669,MYCBP和miR-889-3p之间的相互作用.
在体内和体外功能测试以评估circ_0002669对OS细胞行为的影响.

主要成果:

Circ_0002669在骨组织中表达高,与患者生存率差相关.
升高的circ_0002669促进了OS细胞的增殖和入侵.
Circ_0002669直接与MYCBP结合,抑制其无处不在和蛋白质体降解.
Circ_0002669作为miR-889-3p的miRNA海绵,增加了MYCBP的表达.
确定MYCBP是circ_0002669在OS中的前瘤性作用的关键调解者.
Circ_0002669和MYCBP的过度表达导致c-myc相关基因的表达增加 (CCND1,c-Jun,CDK4).

结论:

Circ_0002669通过稳定MYCBP和抑制miR-889-3p促进骨髓瘤恶性病变.
环_0002669/MYCBP轴代表了骨髓瘤的潜在治疗标.

关键词:

圆周_000266969 在线阅读这是MYCBCBP.骨质肉瘤骨质肉瘤是什么在 miR-889-3p 中使用.

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