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相关概念视频

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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相关实验视频

Updated: Jun 29, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

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基于深度学习的平面场定量相对比显微镜.

Wenjian Wang, Kequn Zhuo, Xin Liu

    Optics express
    |April 4, 2024
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    概括
    此摘要是机器生成的。

    这项研究引入了一种新的定量相对比显微镜 (QPCM) 方法,使用一种新的神经网络和空间光调制器 (SLM). 它只用两张图像实现了高分辨率,无标签的活细胞成像,捕捉了快速的细胞动态.

    更多相关视频

    Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
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    Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

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    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
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    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

    Published on: April 7, 2014

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    相关实验视频

    Last Updated: Jun 29, 2025

    Conducting Multiple Imaging Modes with One Fluorescence Microscope
    08:32

    Conducting Multiple Imaging Modes with One Fluorescence Microscope

    Published on: October 28, 2018

    9.9K
    Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
    09:04

    Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

    Published on: February 23, 2018

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    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
    14:09

    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

    Published on: April 7, 2014

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    科学领域:

    • 生物物理学的生物物理.
    • 细胞生物学 细胞生物学
    • 显微镜的使用方法

    背景情况:

    • 定量相对比显微镜 (QPCM) 提供活细胞的无标签成像,但通常需要多个相位移图像.
    • 现有的QPCM方法在动态细胞过程中面临速度和复杂性的限制.

    研究的目的:

    • 开发一种先进的QPCM技术,减少图像采集要求.
    • 为了提高活细胞成像的速度和效率,用于动态的亚器官行为.

    主要方法:

    • 将一个新的卷积神经网络与QPCM集成,从两个强度图像中重建相位分布.
    • 升级的QPCM设置使用相位式空间光调制器 (SLM) 来一次性记录两个相位移图像.

    主要成果:

    • 在活的COS7和3T3细胞中证明了亚器官 (线粒体,脂质滴) 的高质量成像.
    • 实现了245nm的横向空间分辨率和每秒250 (FPS) 的成像速度.
    • 成功捕捉了细胞结构的快速动态行为实时.

    结论:

    • 拟议的技术可实现高时空分辨率,活细胞的无标签动态成像.
    • 这一进步为研究细胞动态提供了更高效和有效的方法.
    • 该方法对各种生物和生物医学成像应用具有前景.