相关实验视频

Updated: Jun 29, 2025

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

识别长非编码RNAs下调,特别是在卵巢高度血清性癌症

Maki Hayashi-Okada¹, Shun Sato¹, Kengo Nakashima¹

¹Department of Obstetrics and Gynecology Yamaguchi University Graduate School of Medicine Ube Japan.

Reproductive medicine and biology

|April 4, 2024

概括

发现11个长非编码RNAs (lncRNAs) 在卵巢高度血清癌 (HGSC) 中被下调. 像ADAMTS9-AS2,CBR3-AS1和NBR2这样的特定lncRNAs影响了癌细胞的增殖,迁移和入侵.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

卵巢高度血清癌 (HGSC) 是癌症相关死亡的重要原因.
长非编码RNAs (lncRNAs) 在HGSC瘤发生和进展中的作用仍然不完全理解.
识别新的分子标记物和治疗点对于改善HGSC患者的治疗结果至关重要.

研究的目的:

确定与卵巢高度血清癌 (HGSC) 相关的特定长非编码RNA (lncRNAs).
调查这些lncRNAs在HGSC的发展和恶性行为中的参与.
阐明已识别的 lncRNAs 在癌细胞增殖,迁移和入侵中的功能作用.

主要方法:

从HGSC,正常的卵巢和输卵管组织中分离RNA.
使用PCR阵列对84种与癌症相关的lncRNA进行分析.
通过实时RT-PCR验证差异表达的lncRNAs.
通过在卵巢癌细胞系中过度表达选择的lncRNAs进行功能分析,以评估扩散,迁移和入侵.

主要成果:

发现11个lncRNAs,包括ACTA2-AS1,ADAMTS9-AS2,CBR3-AS1,HAND2-AS1,IPW,LINC00312,LINC00887,MEG3,NBR2,TSIX和XIST,与正常组织相比,在HGSC样本中显著下调.
过度表达ADAMTS9-AS2的细胞系显示抑制了增殖,但促进了迁移和入侵.
过度表达CBR3-AS1或NBR2倾向于促进细胞迁移,而在增殖或入侵方面没有显著变化.

结论:

这项研究确定了11个在HGSC中特别下调的lncRNAs.
lncRNAs CBR3-AS1,NBR2和ADAMTS9-AS2在介导HGSC恶性行为的过程中表现出不同的功能作用.
这些发现表明,特定的下调 lncRNAs 可能作为HGSC的潜在生物标志物或治疗点.

关键词:

在 ADAMTS9-AS2 中.在CBR3-AS1中,这就是HGSC.在NBR2中,它是NBR2.在ncRNA中,我们可以看到

更多相关视频

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells

Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells

Published on: June 16, 2023

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...