鉴定和验证骨关节性突纤维细胞中与免疫相关的基因
Yaduan Dai1, Lin Chen1, Zhan Zhang2
1Department of Rehabilitation, Shengjing Hospital of China Medical University, Shenyang, China.
Heliyon
|April 4, 2024
概括
骨关节炎 (OA) 和类风湿性关节炎 (RA) 具有共同的炎症途径. 介素-7受体 (IL7R) 是一个关键的共享基因,突出显示免疫炎症.
科学领域:
- 免疫学 免疫学 免疫学
- 遗传学 是一个遗传学.
- 类风湿病学 类风湿病学
背景情况:
- 骨关节炎 (OA) 越来越多地被认为涉及突关节炎,一种促炎症的疾病.
- 最近的研究表明,OA与类风湿性关节炎 (RA) 共享炎症和代谢途径.
- 调查共享的免疫相关基因和途径可以阐明OA的发病原因.
研究的目的:
- 在OA和RA中识别共免疫相关的基因和途径.
- 探索OA和RA突炎中共享致病的作用.
- 在临床样本中验证已识别的基因的作用.
主要方法:
- 从基因表达综合 (GEO) 数据库下载的基因表达数据集 (GSE29746,GSE12021).
- 在OA和RA群体中使用R软件识别了差异表达基因 (DEGs).
- 使用Venny,Metascape,STRING和Cytoscape进行DEG分析,途径丰富和蛋白质-蛋白质相互作用 (PPI) 网络构建.
- 在临床突样本上使用西部涂抹和实时定量聚合酶连锁反应 (qPCR) 验证的结果.
主要成果:
- 确定了573个OA-DEG,148个RA-DEG和52个OA和RA之间共享的DEG.
- 发现了14个常见的丰富术语,主要与免疫炎症有关.
- 鉴定了干白素-7受体 (IL7R) 作为唯一在OA和RA中升调的共同中心基因.
- 在临床骨关节炎突样本中确认高IL7R表达.
结论:
- IL7R是一种关键的共享基因,在OA和RA中具有差异表达.
- 这些发现证实了免疫炎症在OA和RA病变发生过程中的参与.
- 在RA和OA中,IL7R在突炎症中起作用,需要进一步研究.
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