人类癌症中的巨细胞多样性:通过单细胞分辨率和空间背景提供的新见解
Militsa Rakina1,2, Irina Larionova1,2, Julia Kzhyshkowska1,3,4
1Laboratory of Translational Cellular and Molecular Biomedicine, National Research Tomsk State University, Tomsk, 634050, Russia.
Heliyon
|April 4, 2024
概括
传统的M1/M2巨细胞模型不足以理解与瘤相关的巨细胞 (TAMs). 先进的技术揭示了对癌症进展和治疗反应至关重要的各种TAM子集,提供了新的治疗点.
科学领域:
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
- 基因组学就是基因组学.
背景情况:
- M1/M2巨细胞范式过度简化了巨细胞的可塑性.
- 瘤相关巨细胞 (TAMs) 显著影响瘤进展和治疗结果.
- 对特定TAM状态的有限理解妨碍了有效的治疗向.
研究的目的:
- 总结最近关于人类癌症中新型TAM子集的发现.
- 描述这些TAM子集的转录组特征和临床意义.
- 突出空间转录学在理解TAM相互作用和免疫治疗反应中的作用.
主要方法:
- 单细胞RNA测序以识别TAM子集.
- 空间转录学分析TAM本地化和相互作用.
- 对转录组形状和临床数据的分析.
主要成果:
- 使用先进技术在各种人类癌症中破译了新的功能性TAM子集.
- 具有特征的特定TAM亚群 (例如TREM2+,SPP1+,MARCO+) 与癌症预后相关.
- 空间转录学揭示了TAM,纤维细胞和免疫疗法反应的标记物之间的相互作用.
结论:
- M1/M2二分法不足以描述瘤微环境中的TAM异质性.
- 先进的单细胞和空间技术对于理解TAM功能和临床相关性至关重要.
- 针对特定的TAM子集及其相互作用为新型癌症免疫疗法提供了有希望的途径.
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