相关实验视频

Updated: Jun 29, 2025

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

增加的miR-6132通过降低FOXP3表达的调节促进深静脉血栓形成

Yunhong Zhang¹, Zhen Zhang¹, Haoyang Li²

¹Innovative Institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province, China.

Frontiers in cardiovascular medicine

|April 4, 2024

概括

深静脉血栓症 (DVT) 涉及异常的基因表达. 这项研究表明,增加miR-6132可以降低分叉盒蛋白3 (FOXP3),促进DVT的进展.

科学领域:

血管生物学血管生物学
分子遗传学分子遗传学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

深静脉血栓症 (DVT) 是一种普遍存在的外周血管疾病,与异常的基因表达有关.
异常的表观遗传修饰,特别是分叉盒蛋白3 (FOXP3) 的转录后调节,都与DVT的发病有关.

研究的目的:

研究微RNAs (miRNAs) 在调节DVT中的FOXP3表达中的作用.
阐明miR-6132影响FOXP3水平和DVT发育的特定机制.

主要方法:

微阵列分析来比较miRNA和mRNA在DVT中的表达特征.
双 luciferase 记者测定以确认直接的 miRNA-目标相互作用.
在体内DVT模型使用多普勒超声波和组织学分析来评估miR-6132的功能影响.

主要成果:

在DVT中观察到miR-6132和FOXP3表达之间存在显著的负相关性.
过度表达miR-6132导致FOXP3降低和严重的DVT,而敲击则有相反的效果.
证实了miR-6132与FOXP3的直接结合,这表明了监管关系.

结论:

升高的miR-6132通过抑制FOXP3表达,有助于DVT的形成和进展.
这项研究确定了一种新的表观遗传途径,涉及miR-6132和FOXP3在DVT病变发生过程中.
针对miR-6132/FOXP3轴可能为DVT提供治疗策略.

关键词:

在FOXP3中,我们可以看到FOXP3.深静脉血栓形成的原因是深静脉血栓形成.免疫反应的免疫反应.在 miR-6132 的位置上.转录后监管的规定.

更多相关视频

Deep Vein Thrombosis Induced by Stasis in Mice Monitored by High Frequency Ultrasonography

Deep Vein Thrombosis Induced by Stasis in Mice Monitored by High Frequency Ultrasonography

Published on: April 13, 2018

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...